Rabbit Anti-PUS1 Recombinant Antibody (clone R02-0A2) (CAT#: VS3-FY1862)
Clone R02-0A2 is a rabbit recombinant monoclonal antibody that with high affinity for human PUS1. It cross-reacts with hamster, rat. It is suitable for use in a variety of assays, including WB, ICC/IF, IP.
Recommended dilution is western blot (dilution 1/500-1/1000), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20).
Specific Inquiry
WB
Figure 1 Anti-PUS1 Antibody (VS3-FY1862) in WB.
The PUS1 expression in Jurkat, C6, CHO-K1, Hela lysates were detected using the anti-PUS1 by western blot assay.
Specifications
- Immunogen
- Recombinant human PUS1 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Hamster, Rat PUS1
- Species Reactivity
- Human, Hamster, Rat
- Clone
- R02-0A2
- Applications
- Western Blot, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 47 kDa, while the observed molecular weight stands at 44,47 kDa.
- Related Disease
- Myopathy, Lactic Acidosis, And Sideroblastic Anemia 1 and Myopathy, Lactic Acidosis, And Sideroblastic Anemia
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- MLASA1
- Gene ID
- 80324
- UniProt ID
- Q9Y606
- Sequence Similarities
- Belongs to the tRNA pseudouridine synthase TruA family.
- Cellular Localization
- Cytoplasm, Mitochondrion, Nucleus
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (Q9Y606)
Modification sites at neXtProt (NX_Q9Y606)
Glycosylation from GlyGen (Q9Y606) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001002019.1; NP_001002020.1; NP_079491.2
- Function
- Acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA.
Also catalyzes pseudouridylation of mRNAs: mediates pseudouridylation of mRNAs with the consensus sequence 5'-UGUAG-3'.
Acts as a regulator of pre-mRNA splicing by mediating pseudouridylation of pre-mRNAs at locations associated with alternatively spliced regions.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "PUS1"
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2943 | Hi-Affi™ Recombinant Rabbit Anti-PUS1 Monoclonal Antibody (DS2943AB) | FC, ICC/IF, IHC-P, WB | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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