Rabbit Anti-QSOX1 Recombinant Antibody (clone R06-7A8) (CAT#: VS3-FY2073)

The anti-QSOX1 antibody can be applied to western blot analysis of quiescin Q6 in A549, U2OS lysates. The recombinant monoclonal antibody exhibits selective binding to human QSOX1. It is suitable for use in a variety of assays, including WB, IP.


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WB

Figure 1 Anti-QSOX1 Antibody (VS3-FY2073) in WB.

Figure 1 Anti-QSOX1 Antibody (VS3-FY2073) in WB.

The Quiescin Q6 expression in A549, U2OS lysates were detected using the anti-QSOX1 by western blot assay.


Specifications

  • Immunogen
  • Synthetic peptide of human Quiescin Q6
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human QSOX1
  • Species Reactivity
  • Human
  • Clone
  • R06-7A8
  • Applications
  • Western Blot, Immunoprecipitation
  • MW
  • The calculated weight of the target protein is approximately 83 kDa, while the observed molecular weight stands at 83 kDa.

Product Property

  • Purification
  • Affinity Purified
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
  • Preservative
  • 0.01% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunoprecipitation: 1/20

Target

  • Alternative Names
  • Quiescin Sulfhydryl Oxidase 1; Quiescin Q6 Sulfhydryl Oxidase 1; Thiol Oxidase 1; Quiescin Q6; EC 1.8.3.2; QSCN6
  • Sequence Similarities
  • Belongs to the quiescin-sulfhydryl oxidase (QSOX) family.
  • Cellular Localization
  • Golgi apparatus, Membrane, Secreted
  • Post Translation Modifications
  • N-glycosylated (PubMed:17331072, 29757379).
    O-glycosylated on Thr and Ser residues (PubMed:29757379).
    (O00391-QSOX1_HUMAN)
    Glycosylation at Asn130, Asn243, Thr276, Thr277, Thr281, Thr282, Thr289, Ser493, Ser549, Asn575, Ser576, Thr577, Ser588, Thr590, Asn591, Thr592, Thr593, Ser604, Ser643, Thr647, and Ser655 (NX_O00391 [NX_O00391-1])
    Modification sites at PhosphoSitePlus (O00391)
    Glycosylation from GlyConnect QSOX1_HUMAN (1774)
    Glycosylation from GlyGen (O00391) 22 sites, 30 N-linked glycans (4 sites), 3 O-linked glycans (18 sites)
  • Function
  • Catalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide.
    Plays a role in disulfide bond formation in a variety of extracellular proteins.

Recommended Products

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Recombinant Antibody

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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