Mouse Anti-RAB8A Recombinant Antibody (VS3-CJ1072) (CAT#: VS3-CJ1072)

• Immunogen
• Recombinant protein of RAB8A

• Host Species
• Mouse

• Type
• Mouse IgG1, κ

• Specificity
• Human, Mouse RAB8A

• Species Reactivity
• Human, Mouse

• Applications
• WB

• Conjugate
• Unconjugated

• Purification
• Protein G affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• PBS

• Preservative
• 0.09% Sodium Azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• This antibody has been tested for use in Western Blot (1:1000).

• Alternative Names
• MEL; RAB8

• Gene ID
• 4218

• UniProt ID
• P61006

• Sequence Similarities
• Belongs to the small GTPase superfamily. Rab family.

• Cellular Localization
• Cell membrane, Cell projection, Cilium, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endosome, Golgi apparatus, Membrane

• Post Translation Modifications
• Phosphorylation of Thr-72 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM, CHML and RAB GDP dissociation inhibitors GDI1 and GDI2.

• Protein Refseq
• NP_005361.2

• Function
• The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis (PubMed:20890297).
  Regulates the compacted morphology of the Golgi (PubMed:26209634).
  Together with MYO5B and RAB11A participates in epithelial cell polarization (PubMed:21282656).
  Also involved in membrane trafficking to the cilium and ciliogenesis (PubMed:21844891, PubMed:30398148).
  Together with MICALL2, may also regulate adherens junction assembly (By similarity).
  May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis (By similarity).
  Involved in autophagy (PubMed:27103069).

Secondary Antibody

• 2RD-A1460-YJ
• Goat Anti-Mouse IgG1 Secondary antibody

• 2RD-A1562-YJ
• Rabbit Anti-Mouse IgG1 Secondary antibody (Clone RM106)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS