Rabbit Anti-RAN Recombinant Antibody (clone R01-7J8) (CAT#: VS3-FY1868)
Clone R01-7J8 is a rabbit recombinant monoclonal antibody that exhibits specific detecting to human RAN. The RAN protein has a calculated weight of 24 kDa. It cross-reacts with mouse, monkey.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R01-7J8 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunofluorescence (dilution range 1:50-1:200), immunoprecipitation (dilution range 1:20).
Specific Inquiry
Subcellular Location
RNA Expression
Figure 1 IF staining of human cell line RT4
(Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63353/1392_C11_2_selected.jpg
(Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63353/1392_C11_2_selected.jpg
Figure 2 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000132341-RAN
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000132341-RAN
Specifications
- Immunogen
- Synthetic peptide of human Ran
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Monkey RAN
- Species Reactivity
- Human, Mouse, Monkey
- Clone
- R01-7J8
- Applications
- Western Blot, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 24 kDa, while the observed molecular weight stands at 24 kDa.
- Related Disease
- Kennedy's disease
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- RAN, Member RAS Oncogene Family; Androgen Receptor-Associated Protein 24; Ras-Related Nuclear Protein; Ras-Like Protein TC4; GTPase Ran; ARA24; GTP-Binding Nuclear Protein Ran
- Gene ID
- 5901
- UniProt ID
- P62826
- Sequence Similarities
- Belongs to the small GTPase superfamily. Ran family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Acetylation by KAT5 at Lys-134 is increased during mitosis, impairs RANGRF binding and enhances RCC1 binding (PubMed:29040603).
Acetylation at Lys-37 enhances the association with nuclear export components (PubMed:31075303).
Deacetylation of Lys-37 by SIRT7 regulates the nuclear export of NF-kappa-B subunit RELA/p65 (PubMed:31075303).
- Protein Refseq
- NP_001287725.1; NP_001287726.1; NP_006316.1
- Function
- Acts as a negative regulator of the kinase activity of VRK1 and VRK2.
Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "RAN"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2400z | Mouse Anti-RAN Recombinant Antibody (clone 26D12) | WB, ELISA, ICC, IF, IP | Mouse IgG2b |
| VS3-QX956 | Mouse Anti-RAN Recombinant Antibody (clone A1-6D) | WB, IHC, ICC, FC | Mouse IgG1 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2992 | Hi-Affi™ Recombinant Rabbit Anti-Ran Monoclonal Antibody (DS2992AB) | WB, ICC/IF, IP, FC | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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