Rabbit Anti-RASGRP1 Recombinant Antibody (clone R02-2G4) (CAT#: VS3-FY1959)
The anti-RASGRP1 antibody can be applied to western blot analysis of Rasgrp1 in A549, HL-60, U251 lysates. The recombinant monoclonal antibody shows specific binding to human RASGRP1. The R02-2G4 is recommended for dilution of target protein by western blot (dilution 1/500-1/1000).
Specific Inquiry
WB
Figure 1 Anti-RASGRP1 Antibody (VS3-FY1959) in WB.
The Rasgrp1 expression in A549, HL-60, U251 lysates were detected using the anti-RASGRP1 by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human Rasgrp1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human RASGRP1
- Species Reactivity
- Human
- Clone
- R02-2G4
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 90 kDa, while the observed molecular weight stands at 90 kDa.
- Related Disease
- Immunodeficiency 64 With Lymphoproliferation and Autoimmune Lymphoproliferative Syndrome
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- RAS Guanyl Releasing Protein 1; Calcium- And Diacylglycerol-Regulated Guanine Nucleotide Exchange Factor II; Calcium And DAG-Regulated Guanine Nucleotide Exchange Factor II; RAS Guanyl Releasing Protein 1 (Calcium And DAG-Regulated); CALDAG-GEFII; RASGRP; Guanine Nucleotide Exchange Factor, Calcium- And DAG-Regulated, Rap1A; RAS Guanyl Nucleotide-Releasing Protein 1
- Gene ID
- 10125
- UniProt ID
- O95267
- Cellular Localization
- Cell membrane, Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Membrane
- Post Translation Modifications
- Ubiquitination at Lys596 (NX_O95267 [NX_O95267-1])
Modification sites at PhosphoSitePlus (O95267)
- Protein Refseq
- XP_047288029.1; XP_047288030.1; XP_047288031.1; XP_047288032.1; XP_047288033.1
- Function
- Functions as a calcium- and diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound GDP for GTP.
Activates the Erk/MAP kinase cascade.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "RASGRP1"
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3010 | Hi-Affi™ Recombinant Rabbit Anti-RASGRP1 Monoclonal Antibody (DS3010AB) | WB | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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