Mouse Anti-S100A4 Recombinant Antibody (clone ABT-S100A4) (CAT#: VS3-CJ831)
This product is a mouse antibody that recognizes human S100A4.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A549
(Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7486/2064_B12_5_selected.jpg
(Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7486/2064_B12_5_selected.jpg
Figure 2 Lung.
(High expression in lung)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68227/152283_A_1_4_val_selected.jpg
(High expression in lung)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68227/152283_A_1_4_val_selected.jpg
Figure 3 Spleen
(High expression in spleen)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7973/106662_B_7_4_val_selected.jpg
(High expression in spleen)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7973/106662_B_7_4_val_selected.jpg
Figure 4 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7973/106662_A_7_3.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7973/106662_A_7_3.jpg
Figure 5 Testis
(Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7973/106662_A_6_6.jpg
(Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7973/106662_A_6_6.jpg
Figure 6 Placenta
(Decidual cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Trophoblastic cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7973/106662_A_2_7.jpg
(Decidual cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Trophoblastic cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7973/106662_A_2_7.jpg
Figure 7 Lymph node
(Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27387/106652_A_9_8.jpg
(Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27387/106652_A_9_8.jpg
Figure 8 RNA cell line category: Cell line enhanced (A549, ASC diff, BJ hTERT+, fHDF/TERT166, RPMI-8226)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196154-S100A4
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196154-S100A4
Specifications
- Immunogen
- Synthesized peptide derived from human S100A4
- Host Species
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- Human S100A4
- Species Reactivity
- Human
- Clone
- ABT-S100A4
- Applications
- IHC-P
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity Chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.5% BSA, PBS.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Immunohistochemistry-Parraffin (1:100-1:500).
Target
- Alternative Names
- 42A; 18A2; CAPL; FSP1; MTS1; P9KA; PEL98
- Gene ID
- 6275
- UniProt ID
- P26447
- Sequence Similarities
- Belongs to the S-100 family.
- Cellular Localization
- Cytoplasm, Nucleus, Secreted
- Protein Refseq
- NP_002952.1; NP_062427.1
- Function
- Calcium-binding protein that plays a role in various cellular processes including motility, angiogenesis, cell differentiation, apoptosis, and autophagy (PubMed:16707441, PubMed:23752197, PubMed:30713770).
Increases cell motility and invasiveness by interacting with non-muscle myosin heavy chain (NMMHC) IIA/MYH9 (PubMed:16707441).
Mechanistically, promotes filament depolymerization and increases the amount of soluble myosin-IIA, resulting in the formation of stable protrusions facilitating chemotaxis (By similarity).
Modulates also the pro-apoptotic function of TP53 by binding to its C-terminal transactivation domain within the nucleus and reducing its protein levels (PubMed:23752197).
Within the extracellular space, stimulates cytokine production including granulocyte colony-stimulating factor and CCL24 from T-lymphocytes (By similarity).
In addition, stimulates T-lymphocyte chemotaxis by acting as a chemoattractant complex with PGLYRP1 that promotes lymphocyte migration via CCR5 and CXCR3 receptors (PubMed:30713770, PubMed:26654597).
Recommended Products
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "S100A4"
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-546CL | Human Anti-S100A4 Recombinant Antibody (TAB-546CL) | ELISA | Human IgG |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0135CL-F(E) | Mouse Anti-S100A4 Recombinant Antibody; Fab Fragment (TAB-0135CL-F(E)) | WB, ELISA, BI, Block, Inhib | Mouse Fab |
| TAB-379CT | Mouse Anti-S100A4 Recombinant Antibody (TAB-379CT) | WB, IHC, Block | Mouse IgG2a |
| TAB-380CT | Mouse Anti-S100A4 Recombinant Antibody (TAB-380CT) | WB, IHC, Block | Mouse IgG1 |
| TAB-381CT | Mouse Anti-S100A4 Recombinant Antibody (TAB-381CT) | WB, IHC, Block | Mouse IgG1 |
| TAB-382CT | Mouse Anti-S100A4 Recombinant Antibody (TAB-382CT) | WB, IHC, Block | Mouse IgG1 |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-377CT | Anti-Human S100A4 Therapeutic Single Domain Antibody (aMTS1) | WB, ELISA | Single domain antibody |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-378CT-F(E) | Anti-Human S100A4 Recombinant Antibody Fab Fragment (5C3) | WB, IHC, Block | |
| MOB-1451MZ | Recombinant Mouse Anti-Human S100A4 Antibody (clone 1F12-1G7) | ELISA, ICC/IF, WB | Mouse antibody |
| MRO-1358-CN | Recombinant Rabbit Anti-S100A4 Monoclonal Antibody (CBACN-494) | WB, IF, IHC, IP, FC | Rabbit IgG |
| VS3-CJ832 | Mouse Anti-S100A4 Recombinant Antibody (clone PT0232) | IHC-P | Mouse IgG3, κ |
| VS3-CJ833 | Rabbit Anti-S100A4 Recombinant Antibody (VS3-CJ833) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3155 | Hi-Affi™ Recombinant Rabbit Anti-S100A4 Monoclonal Antibody (DS3155AB) | WB, IHC, ICC, IP | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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