Mouse Anti-SEB Recombinant Antibody (clone pComb-49) (CAT#: VS-0323-CJ50)
This mouse anti-SEB antibody (clone pComb-49) is a product that recognizes Staphylococcal enterotoxin B (SEB).
Specific Inquiry
ELISA
Figure 1 ELISA analysisof the VS-0323-CJ50 (Fab fragment) reacted with SEB and that the staining level was increased in a dose-dependent manner.
Serially diluted Fab fragment was poured onto SEB-coated (•) or uncoated (○) polystylene plates. After washing, the plate Fab fragment was detected by using HRP-labeled protein L.
SPR
Figure 2 SPR analysis of SEB binding VS-0323-CJ50 Fab fragment.
SEB was covalently immobilized by amine coupling to the dextran matrix of a sensor chip CM3 to yield 1,200 resonance units. Followed by blocking with ethanol amine, serially diluted Fab fragment was introduced to the chip at a flow rate of 30 μg/min, and the responses were recorded.
ELISA
Figure 3 ELISA analysisof the VS-0323-CJ50 (scFv fragment) reacted with SEB and that the staining level was increased in a dose-dependent manner.
Serially diluted scFv fragment was poured onto SEB-coated (•) or uncoated (○) polystylene plates. After washing, the plate scFv fragment was detected by using HRP-labeled protein L.
SPR
Figure 4 Serially diluted scFv were introduced to sensorchip-immobilized SEB.
WB
Figure 5 WB analysis of the binding specificity of VS-0323-CJ50.
E. coli cell lysates containing N15-MBP, 1 μg of BSA, and 1 μg of SEB were separated by SDS-PAGE and subjected to Western blot analysis with VS-0323-CJ50 antibody for primary detection.
Specifications
- Immunogen
- Maltose-binding protein (MBP) fused with the N-terminal 15-mer peptide of SEB (N15-MBP protein)
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Staphylococcal enterotoxin B (SEB)
- Species Reactivity
- S. aureus
- Clone
- pComb-49
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Surface Plasmon Resonance
- Conjugate
- Unconjugated
- Related Disease
- Food poisoning
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- ELISA: 0.5-1 μg/well
Western Blot: 1-10 μg/well
Surface Plasmon Resonance: 12 nM-30 nM
Target
- Alternative Names
- Staphylococcal enterotoxin B
- UniProt ID
- P01552
- Sequence Similarities
- Belongs to the staphylococcal/streptococcal toxin family.
- Cellular Localization
- Secreted
- Protein Refseq
- WP_000278085.1
- Function
- Staphylococcal enterotoxin that activates the host immune system by binding as unprocessed molecules to major histocompatibility (MHC) complex class II and T-cell receptor (TCR) molecules. In turn, this ternary complex activates a large number of T-lymphocytes initiating a systemic release of pro-inflammatory cytokines (PubMed:2303780, PubMed:25015819).
Causes also the intoxication staphylococcal food poisoning syndrome (By similarity).
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "SEB"
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-895-S(P) | Recombinant Anti-SEB Antibody scFv Fragment | ELISA, WB, FC, FuncS | scFv |
| PSBL-698 | Recombinant Mouse Anti-Staphylococcal enterotoxin B (SEB) Antibody scFv Fragment (14G8) | WB, ELISA, FuncS | scFv |
| PSBL-699 | Recombinant Mouse Anti-Staphylococcal enterotoxin B (SEB) Antibody scFv Fragment (6D3) | WB, IF, FuncS | scFv |
| PSBL-700 | Recombinant Mouse Anti-Staphylococcal enterotoxin B (SEB) Antibody scFv Fragment (3E2) | ELISA, WB | scFv |
| HPAB-0090-FY-S(P) | Recombinant Mouse Anti-SEB Antibody scFv Fragment (FMU-SEB-No.2) | E |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-336 | Recombinant Human Anti-Staphylococcal enterotoxin B (SEB) Antibody Fab Fragment (3E2) | WB, ELISA, Neut, FuncS | Fab |
| PFBL-337 | Recombinant Mouse Anti-Staphylococcal enterotoxin B (SEB) Antibody Fab Fragment (20B1) | Neut, FuncS | Fab |
| PFBL-698 | Recombinant Mouse Anti-Staphylococcal enterotoxin B (SEB) Antibody Fab Fragment (14G8) | WB, ELISA, FuncS | Fab |
| PFBL-699 | Recombinant Mouse Anti-Staphylococcal enterotoxin B (SEB) Antibody Fab Fragment (6D3) | WB, IF, FuncS | Fab |
| FAMAB-0254-CN-F(E) | Recombinant Mouse Anti-SEB Antibody Fab Fragment (2F6D9G2) | WB, ELISA | Mouse Fab |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PNBL-029 | Recombinant Anti-Staphylococcal SEB VHH Single Domain Antibody (PNBL-029) | SPR | Llama VHH |
| PNBL-094 | Recombinant Anti-Staphylococcal enterotoxin B (SEB) VHH Single Domain Antibody (PNBL-094) | WB, FuncS | Llama VHH |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0872CT | Recombinant Mouse anti-Staphylococcus aureus seb Monoclonal antibody (T333) | sELISA | |
| FAMAB-0327WJ | Camelid Anti-SEB Recombinant Antibody (clone LSEBg6) | ELISA, Neut | Camelid VHH |
| FAMAB-0328WJ | Camelid Anti-SEB Recombinant Antibody (clone LSEBA6) | ELISA, Neut | Camelid VHH |
| FAMAB-0329WJ | Camelid Anti-SEB Recombinant Antibody (clone LSEBe3) | ELISA, Neut | Camelid VHH |
| FAMAB-0330WJ | Camelid Anti-SEB Recombinant Antibody (clone B12) | ELISA, WB, Neut | Camelid VHH |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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