Rabbit Anti-SF3A3 Recombinant Antibody (clone R06-3I7) (CAT#: VS3-FY1944)

Clone R06-3I7 is a rabbit recombinant monoclonal antibody that has a high affinity for human SF3A3. It cross-reacts with mouse, rat, hamster. Applications Tested: The SF3A3 antibody has been tested by western blot analysis of SF3A3 in 3T3, Raw264.7, C6 lysates.


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WB

Figure 1 Anti-SF3A3 Antibody (VS3-FY1944) in WB.

Figure 1 Anti-SF3A3 Antibody (VS3-FY1944) in WB.

The SF3A3 expression in 3T3, Raw264.7, C6 lysates were detected using the anti-SF3A3 by western blot assay.


Specifications

  • Immunogen
  • Synthetic peptide of human SF3A3
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse, Rat, Hamster SF3A3
  • Species Reactivity
  • Human, Mouse, Rat, Hamster
  • Clone
  • R06-3I7
  • Applications
  • Western Blot
  • MW
  • The calculated weight of the target protein is approximately 59 kDa, while the observed molecular weight stands at 59 kDa.

Product Property

  • Purification
  • Affinity Purified
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000

Target

  • Alternative Names
  • Splicing Factor 3a Subunit 3; Splicing Factor 3a, Subunit 3, 60kDa; Splicing Factor 3a, Subunit 3, 60kD; SAP 61; SF3a60; SAP61
  • Sequence Similarities
  • Belongs to the SF3A3 family.
  • Cellular Localization
  • Nucleus, Spliceosome
  • Post Translation Modifications
  • Ubiquitination at Lys201 and Lys390 (NX_Q12874 [NX_Q12874-1])
    Modification sites at PhosphoSitePlus (Q12874)
    Glycosylation from GlyGen (Q12874) 2 sites, 1 O-linked glycan (2 sites)
  • Function
  • The 17S U2 SnRNP complex 1 directly participates in early spliceosome assembly and 2 mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing.

Recommended Products

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Recombinant Antibody

Rabbit Monoclonal Antibody

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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