Rabbit Anti-SOD1 Recombinant Antibody (clone 3E5) (CAT#: ZG-0574U)

Rabbit Anti-SOD1 Antibody (clone 3E5) displayed high specificity against the SOD1 protein of Human.


Specific Inquiry
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  • Tested Data
  • Gene Expression
  • Datasheet
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  • COA
IHC

Figure 1 Rabbit Anti-SOD1 Antibody (ZG-0574U) in IHC

Figure 1 Rabbit Anti-SOD1 Antibody (ZG-0574U) in IHC

Western Blot
Positive WB detected in: 293 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, U87 whole cell lysate
All lanes: SOD1 antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 16 kDa
Observed band size: 18 kDa

IHC

Figure 2 Rabbit Anti-SOD1 Antibody (ZG-0574U) in IHC

Figure 2 Rabbit Anti-SOD1 Antibody (ZG-0574U) in IHC

Overlay histogram showing Jurkat cells stained with ZG-0574U (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of>10, 000 events was performed.


Specifications

  • Immunogen
  • A synthesized peptide derived from human SOD1
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human SOD1
  • Species Reactivity
  • Human
  • Clone
  • 3E5
  • Applications
  • ELISA, WB, FC
  • Conjugate
  • Unconjugated
  • Related Disease
  • Amyotrophic Lateral Sclerosis 1 and Spastic Tetraplegia And Axial Hypotonia, Progressive.

Product Property

  • Purification
  • Affinity chromatography purified
  • Buffer
  • PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Preservative
  • 0.02% sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry. Specific conditions should be optimized by the end user.
    WB:1:500-1:5000, FC:1:20-1:200

Target

  • Alternative Names
  • Superoxide dismutase [Cu-Zn]; EC 1.15.1.1; Superoxide dismutase 1; hSod1; SOD1

Related Resources

  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "SOD1"

Recombinant Antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-3369 Hi-Affi™ Recombinant Rabbit Anti-SOD1 Monoclonal Antibody (DS3369AB) ICC/IF, IHC-P, IP, WB IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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