Mouse Anti-Staphylococcal enterotoxin B (SEB) Recombinant Antibody (Clone 4PCL2) (CAT#: PABS-0023)

This recombinant monoclonal antibody to Staphylococcal enterotoxin B (SEB) is a mouse monoclonal antibody that can be used for applications: ELISA, SPR.


Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
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  • Published Data
  • Datasheet
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  • COA
ELISA

Figure 1 ELISA showing binding of antiSEB ScFv with SEB antigen.

Figure 1 ELISA showing binding of antiSEB ScFv with SEB antigen.

ELISA was performed with different dilutions of soluble anti-SEB ScFv antibody. Periplasmic extract of untransformed HB2151 cells was used as a negative control (OD, 0.0945). The bound anti-SEB ScFv antibody was detected using anti-E-tag HRP-conjugated antibody.

Singh, P. K., Agrawal, R., Kamboj, D. V., Gupta, G., Boopathi, M., Goel, A. K., & Singh, L. (2010). Construction of a single-chain variable-fragment antibody against the superantigen staphylococcal enterotoxin B. Applied and environmental microbiology, 76(24), 8184-8191.

WB

Figure 2 Western blot showing binding of antiSEB ScFv with SEB antigen.

Figure 2 Western blot showing binding of antiSEB ScFv with SEB antigen.

Lane 1, prestained molecular mass markers (70, 55, 35, 27, and 15 kDa); lane 2, r-SEB band at 30.5 kDa showing binding with anti-SEB ScFv. The bound anti-SEB ScFv antibody was detected using anti-E-tag HRP-conjugated antibody.

Singh, P. K., Agrawal, R., Kamboj, D. V., Gupta, G., Boopathi, M., Goel, A. K., & Singh, L. (2010). Construction of a single-chain variable-fragment antibody against the superantigen staphylococcal enterotoxin B. Applied and environmental microbiology, 76(24), 8184-8191.


Specifications

  • Host Species
  • Mouse
  • Derivation
  • Phage display library
  • Type
  • Mouse IgG
  • Specificity
  • Staphylococcal enterotoxin B (SEB)
  • Species Reactivity
  • S. aureus
  • Clone
  • 4PCL2
  • Applications
  • ELISA, SPR, FuncS
  • Related Disease
  • Gastroenteritis

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • staphylococcal enterotoxin B; SEB

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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