Mouse Anti-Tau Recombinant Antibody (clone Tau-5) (CAT#: FAMAB-1513CQ)

Figure 1 Tau-5 immunostaining of rat interfascicular oligodendrocytes, in situ.

PLP-fixed vibratome sections (10 um) from the corpus callosum of the rat (A, low power; B, high power). (Bars = 10 um.) Note the intense staining of the cytoplasm in the cell bodies and the absence of nuclear staining. Also note that Tau-5 does not stain axons under these fixation conditions. (Inset) Ultrastructural localization of Tau-5 processed from a similar section of corpus collosum.

LoPresti, P., Szuchet, S., Papasozomenos, S. C., Zinkowski, R. P., & Binder, L. (1995). Functional implications for the microtubule-associated protein tau: localization in oligodendrocytes. Proceedings of the National Academy of Sciences, 92(22), 10369-10373.

Figure 2 Staining of OLGs with Tau-5.

Four-week-old cultured OLGs as seen by Tau-5 immunofluorescence (A) and by phase contrast microscopy (B). (Bar = 20 um.) OLGs show strong cytoplasmic staining (A).

LoPresti, P., Szuchet, S., Papasozomenos, S. C., Zinkowski, R. P., & Binder, L. (1995). Functional implications for the microtubule-associated protein tau: localization in oligodendrocytes. Proceedings of the National Academy of Sciences, 92(22), 10369-10373.

Figure 3 Immunoblot analysis of tau in cultured OLGs using three mAbs to distinct epitopes.

Many isoforms of tau can be visualized in a Tau-1 blot of lamb cortex (lane 1), whereas a different pattern of isoforms is visualized in cultured OLGs whether the blot is probed with Tau-1 (lane 2), Tau-5 (lane 3), or Tau46 (lane 4).

LoPresti, P., Szuchet, S., Papasozomenos, S. C., Zinkowski, R. P., & Binder, L. (1995). Functional implications for the microtubule-associated protein tau: localization in oligodendrocytes. Proceedings of the National Academy of Sciences, 92(22), 10369-10373.