Rabbit Anti-TBX21 Recombinant Antibody (clone R07-7Y4) (CAT#: VS3-XY3520)
The recombinant rabbit antibody is specific for TBX21 (T-box transcription factor 21), which activates transcription of a set of genes important for Th1 cell function. TBX21 also inhibits the Th17 cell lineage commitment by blocking RUNX1-mediated transactivation of Th17 cell-specific transcriptinal regulator RORC. The antibody specifically reacts with human TBX21. It has been tested using Immunohistochemistry-Paraffin and Flow cytometry. The recommended dilutions of Immunohistochemistry-Paraffin and Flow cytometry is 1/50-1/100.
Specific Inquiry
Normal Tissue
RNA Expression
Figure 1 Spleen
(High expression)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9524/24853_B_7_4_val_selected.jpg
(High expression)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9524/24853_B_7_4_val_selected.jpg
Figure 2 Liver
(Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30043/70685_A_8_4.jpg
(Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30043/70685_A_8_4.jpg
Figure 3 Bone marrow
(Hematopoietic cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30043/70685_B_5_4.jpg
(Hematopoietic cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30043/70685_B_5_4.jpg
Figure 4 Lymph node
(Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30043/70685_A_9_8.jpg
(Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30043/70685_A_9_8.jpg
Figure 5 RNA cell line category: Cell line enriched (HDLM-2)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000073861-TBX21
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000073861-TBX21
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human TBX21
- Species Reactivity
- Human
- Clone
- R07-7Y4
- Applications
- IHC-P, FC
- Related Disease
- Asthma; Nasal Polyps; Aspirin Intolerance; Immunodeficiency 88
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- IHC: 1/50-1/100
FC: 1/50-1/100
Target
- Alternative Names
- TBET; IMD88; T-PET; T-bet; TBLYM
- Gene ID
- 30009
- UniProt ID
- Q9UL17
- Long Name
- T-box transcription factor 21
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Phosphorylations at Ser-53, Tyr-77, Ser-225 and Ser-513 are regulated by mTORC1.
Phosphorylation at Tyr-530 is essential for its interaction GATA3.
Phosphorylation at Tyr-220, Tyr-266 and Tyr-305 enhances its transcriptional activator activity.
Phosphorylation at Thr-303 is required for its interaction with NFATC2.
- Protein Refseq
- NP_037483.1
- Function
- Lineage-defining transcription factor which initiates Th1 lineage development from naive Th precursor cells both by activating Th1 genetic programs and by repressing the opposing Th2 and Th17 genetic programs (PubMed:10761931).
Activates transcription of a set of genes important for Th1 cell function, including those encoding IFN-gamma and the chemokine receptor CXCR3.
Induces permissive chromatin accessibilty and CpG methylation in IFNG (PubMed:33296702).
Activates IFNG and CXCR3 genes in part by recruiting chromatin remodeling complexes including KDM6B, a SMARCA4-containing SWI/SNF-complex, and an H3K4me2-methyltransferase complex to their promoters and all of these complexes serve to establish a more permissive chromatin state conducive with transcriptional activation.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Related Resources
Inflammatory Bowel Diseases
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "TBX21"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3561z | Mouse Anti-TBX21 Recombinant Antibody (clone 29A4) | WB, IP, IF, ELISA | Mouse IgG1, κ |
| MOB-1929CT | Recombinant Mouse anti-Human TBX21 Monoclonal antibody (39D) | ELISA, WB | |
| MRO-2210-CN | Rabbit Anti-Tbx21 Polyclonal Antibody (MRO-2210-CN) | WB, FC | Rabbit IgG |
Intrabody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B024(A) | Recombinant Anti-human TBX21 Intrabody [(D-Arg)9] | CO-IP, FuncS | scFv-(D-Arg)9 |
| IAB-B024(G) | Recombinant Anti-human TBX21 Intrabody [+36 GFP] | ELISA, IF, Neut, FuncS | scFv-(+36GFP) |
| IAB-B024(T) | Recombinant Anti-human TBX21 Intrabody [Tat] | ELISA, Neut, FuncS | scFv-Tat |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3514 | Rabbit Anti-TBX21 Recombinant Antibody (clone DS3514AB) | ELISA | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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