Mouse Anti-TIM-3 Recombinant Antibody (clone 2C12) (CAT#: FAMAB-1303CQ)
This product is a recombinant mouse antibody that recognizes TIM-3. The antibody was purified by affinity chromatography. It can be used in Block, FuncS, FC, IF, IHC.
Specific Inquiry
FuncS
Figure 1 Anti-murine Tim-3 do not deplete Tim-3+ cells in vivo.
Total number of CD4+ and CD8+ cells in treated mice. Error bars represent s.e.m.
Sabatos-Peyton, C. A., Nevin, J., Brock, A., Venable, J. D., Tan, D. J., Kassam, N., ... & Acharya, N. (2018). Blockade of Tim-3 binding to phosphatidylserine and CEACAM1 is a shared feature of anti-Tim-3 antibodies that have functional efficacy. Oncoimmunology, 7(2), e1385690.
ELISA
Figure 2 Anti-murine Tim-3 antibodies do not block galectin-9 binding.
mTim-3-Ig was incubated with 2C12 prior to addition to ELISA plates coated with galectin-9.
Sabatos-Peyton, C. A., Nevin, J., Brock, A., Venable, J. D., Tan, D. J., Kassam, N., ... & Acharya, N. (2018). Blockade of Tim-3 binding to phosphatidylserine and CEACAM1 is a shared feature of anti-Tim-3 antibodies that have functional efficacy. Oncoimmunology, 7(2), e1385690.
FC
Figure 3 Effect of anti-murine Tim-3 antibodies on binding to phosphatidylserine.
mTim-3-Ig was incubated with 2C12 prior to addition to dexamethasone treated thymocytes. Data are representative of 4 independent experiments.
Sabatos-Peyton, C. A., Nevin, J., Brock, A., Venable, J. D., Tan, D. J., Kassam, N., ... & Acharya, N. (2018). Blockade of Tim-3 binding to phosphatidylserine and CEACAM1 is a shared feature of anti-Tim-3 antibodies that have functional efficacy. Oncoimmunology, 7(2), e1385690.
Specifications
- Immunogen
- TIM-3:Ig fusion protein
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Mouse TIM-3
- Species Reactivity
- Mouse
- Clone
- 2C12
- Applications
- FuncS, ELISA, FC
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Function Assay, Flow Cytometry and Enzyme-Linked Immunosorbent Assay.
Target
- Alternative Names
- T-cell immunoglobulin domain and mucin domain-containing molecule-3; Tim-3
- UniProt ID
- Q8VIM0
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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