Rabbit Anti-TIMP1 Recombinant Antibody (clone R03-9H5) (CAT#: VS3-XY3526)
This is a recombinant rabbit antibody targeting the TIMP1 (TIMP metallopeptidase inhibitor 1), which plays a role in integrin signaling. The antibody specifically recognizes human TIMP1. It has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Western blot, Immunohistochemistry-Paraffin and Immunofluorescence is 1/500-1/1000, 1/50-1/100 and 1/50-1/200, respectively.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22360/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22360/if_selected.jpg
Figure 2 IHC staining of human prostate
(Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/53417/ihc_selected.jpg
(Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/53417/ihc_selected.jpg
Figure 3 Kidney
(Cells in tubules Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/53417/139501_A_7_5.jpg
(Cells in tubules Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/53417/139501_A_7_5.jpg
Figure 4 Prostate
(Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/53417/139501_A_2_5.jpg
(Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/53417/139501_A_2_5.jpg
Figure 5 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, fHDF/TERT166, HSkMC)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000102265-TIMP1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000102265-TIMP1
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human TIMP1
- Species Reactivity
- Human
- Clone
- R03-9H5
- Applications
- WB, IHC-P, ICC, IF
- Related Disease
- Oral Submucous Fibrosis; Conjunctivochalasis
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
Target
- Alternative Names
- EPA; EPO; HCI; CLGI; TIMP; TIMP-1
- Gene ID
- 7076
- UniProt ID
- P01033
- Long Name
- TIMP metallopeptidase inhibitor 1
- Cellular Localization
- Secreted
- Post Translation Modifications
- The activity of TIMP1 is dependent on the presence of disulfide bonds. (P01033-TIMP1_HUMAN)
N-glycosylated. (P01033-TIMP1_HUMAN)
Glycosylation at Asn53 and Asn101 (NX_P01033 [NX_P01033-1])
Modification sites at PhosphoSitePlus (P01033)
- Protein Refseq
- NP_003245.1
- Function
- Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor.
Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16.
Does not act on MMP14.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "TIMP1"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1378z | Mouse Anti-TIMP1 Recombinant Antibody (clone 39D12) | WB, ELISA | Mouse IgG1 |
| ZG-0125C | Mouse Anti-TIMP1 Recombinant Antibody (clone PT2236) | IHC-P, IF | Mouse IgG |
| VS3-CJ224 | Mouse Anti-TIMP1 Recombinant Antibody (VS3-CJ224) | IHC | Mouse IgG1 |
| VS3-CJ225 | Mouse Anti-TIMP1 Recombinant Antibody (VS3-CJ225) | IHC | Mouse IgG1 |
| VS-0923-FY146 | Recombinant Mouse Anti-TIMP1 Antibody (VS-0923-FY146) | ELISA, WB | Mouse IgG1 |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-683CT | Anti-Human TIMP-1 Recombinant Antibody (MS-BW-3 ) | ELISA, BL | Human antibody |
| TAB-684CT | Anti-Human TIMP-1 Recombinant Antibody (MS-BW-28) | ELISA, BL | Human antibody |
| TAB-685CT | Anti-Human TIMP-1 Recombinant Antibody (MS-BW-1) | ELISA, BL | Human antibody |
| TAB-686CT | Anti-Human TIMP-1 Recombinant Antibody (MS-BW-27) | ELISA, BL | Human antibody |
| TAB-683CT-S(P) | Anti-Human TIMP-1 Recombinant Antibody scFv Fragment (MS-BW-3 ) | ELISA, BL | Human antibody |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3575 | Rabbit Anti-TIMP1 Recombinant Antibody (clone DS3575AB) | WB | Rabbit IgG |
| MOR-4458 | Rabbit Anti-TIMP1 Recombinant Antibody (clone SI552DS) | ELISA | Rabbit IgG |
MHC Tetramer for Cancer
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC1976 | PE-B7/Human TIMP1 (APFEPLASGIL) MHC Tetramer | FCM |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0174-YC-S(P) | Human Anti-TIMP1 Recombinant Antibody (clone HuCAL); scFv Fragment | ELISA | Human scFv |
| HPAB-0192-WJ-S(P) | Human Anti-TIMP1 Recombinant Antibody; scFv Fragment (HPAB-0192-WJ-S(P)) | ELISA | Human scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0174-YC-F(E) | Human Anti-TIMP1 Recombinant Antibody (clone HuCAL); Fab Fragment | ELISA | Human Fab |
| HPAB-0192-WJ-F(E) | Human Anti-TIMP1 Recombinant Antibody; Fab Fragment (HPAB-0192-WJ-F(E)) | ELISA | Human Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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