Mouse anti-TNFRSF17 antibody (clone 10A9F7) displayed high specificity against the TNFRSF17 protein of Human.
Figure 1 Mouse Anti-TNFRSF17 Antibody (ZG-0264U) in WB
Positive WB detected in: 1-2 lanes: 293F whole cell lysate transfected with BCMA; 3-4 lane: Recombinant proteins with BCMA. All lanes: TNFRSF17 antibody at 1: 1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 40, 15 KDa
Observed band size: 40, 15 KDa
Exposure time: 10min
Figure 2 Mouse Anti-TNFRSF17 Antibody (ZG-0264U) in IF
Immunofluorescence staining of 293F cells transfected with TNFRSF17 with ZG-0264U at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was R-PE-conjugated Goat Anti-Mouse IgG(H+L).
Figure 3 Mouse Anti-TNFRSF17 Antibody (ZG-0264U) in FC
Overlay histogram showing 293F cells transfected with TNFRSF17 stained with ZG-0264U (red line) at 1:100. The cells were fixed in 70% ethanol at 4°C overnight. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was Alexa Fluor 647 Donkey Anti-Mouse IgG (H+L) at 1/250 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1620z | Mouse Anti-TNFRSF17 Recombinant Antibody (clone 2A10) | WB, ELISA, FC | Mouse IgG1 |
PABL-019 | Mouse Anti-TNFRSF17 Recombinant Antibody (clone J22.9-xi) | ELISA, FC, Neut, FuncS | Mouse IgG |
HPAB-0009-DJB | Mouse Anti-TNFRSF17 Recombinant Antibody (clone CA8) | ELISA, Neut, FC, SPR, ADCC, FuncS | Mouse IgG |
HPAB-0010-DJB | Human Anti-TNFRSF17 Recombinant Antibody (HPAB-0010-DJB) | ELISA, Neut, ADCC | Humanized IgG |
HPAB-0011-DJB | Mouse Anti-TNFRSF17 Recombinant Antibody (HPAB-0011-DJB) | ELISA, FC, IP | Mouse IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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