Rabbit Anti-TPBG Recombinant Antibody (clone 6E9) (CAT#: ZG-0047U)

The purified Rabbit Anti-TPBG Recombinant Antibody (clone 6E9) displayed high specificity against the TPBG protein of human and mouse.


Specific Inquiry
  • Size:
  • Conjugation:
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  • Purity:
  • Fc Engineering:
  • Tested Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
WB

Figure 1 Rabbit Anti-TPBG Antibody (ZG-0047U) in WB

Figure 1 Rabbit Anti-TPBG Antibody (ZG-0047U) in WB

Positive WB detected in: U-87 whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysate, U-251 whole cell lysate, Mouse Brain whole cell lysate. All lanes: 5T4 antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 47 kDa
Observed band size: 50, 80 kDa

IHC

Figure 2 Rabbit Anti-TPBG Antibody (ZG-0047U) in IHC

Figure 2 Rabbit Anti-TPBG Antibody (ZG-0047U) in IHC

IHC image of ZG-0047U diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.


Specifications

  • Immunogen
  • A synthesized peptide derived from human 5T4
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human TPBG
  • Species Reactivity
  • Human, Mouse
  • Clone
  • 6E9
  • Applications
  • ELISA, WB, IHC
  • Conjugate
  • Unconjugated
  • Related Disease
  • Congenital Disorder Of Glycosylation, Type Il and Renal Cell Carcinoma, Nonpapillary

Product Property

  • Purification
  • Affinity purified
  • Concentration
  • See the COA
  • Buffer
  • PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Preservative
  • 0.02% sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, western blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
    WB:1:500-1:5000, IHC:1:50-1:200

Target

  • Alternative Names
  • Trophoblast glycoprotein; 5T4 oncofetal antigen; 5T4 oncofetal trophoblast glycoprotein; 5T4 oncotrophoblast glycoprotein; M6P1; Wnt-activated inhibitory factor 1; WAIF1; TPBG; 5T4

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "TPBG"

Mouse Antibody

CAT Product Name Application Type
TAB-118-F(E) Anti-Human 5T4 Recombinant Antibody Fab Fragment, 5T4 (Naptumomab Estafenatox) FuncS, IF, Neut, ELISA, FC, IP, ICC Fab - G1 - kappa

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-3649 Rabbit Anti-TPBG Recombinant Antibody (clone DS3649AB) WB, ICC, IP Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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