Human Anti-Trastuzumab Recombinant Antibody (clone scFv69) (CAT#: FAMAB-1416CQ)

Figure 1 ELISA of pooledsera from mice (n= 20/group) immunized with scFv40, scFv69 or HER2-Fc.

Values are presented as mean ± SD of the Absorbance at 490 nm from three independent experiments.

Ladjemi, M. Z., Chardes, T., Corgnac, S., Garambois, V., Morisseau, S., Robert, B., ... & Pelegrin, A. (2011). Vaccination with human anti-trastuzumab anti-idiotype scFv reverses HER2 immunological tolerance and induces tumor immunity in MMTV. f. huHER2 (Fo5) mice. Breast Cancer Research, 13(1), R17.

Figure 2 Flow cytometry analysis in HER2-overexpressing SK-OV-3 of the HER2-specific response of pooled sera from mice immunized with PBS, anti-Id scFv40, scFv69 or with HER2-Fc.

Sera were recovered at Day 42 post-vaccination. Antibody binding was detected using a FITC-labeled goat anti-mouse antibody. FITC-labeled secondary antibody alone was used asa negative control to determine the level of background fluorescence.

Ladjemi, M. Z., Chardes, T., Corgnac, S., Garambois, V., Morisseau, S., Robert, B., ... & Pelegrin, A. (2011). Vaccination with human anti-trastuzumab anti-idiotype scFv reverses HER2 immunological tolerance and induces tumor immunity in MMTV. f. huHER2 (Fo5) mice. Breast Cancer Research, 13(1), R17.

Figure 3 Inhibition of cell viability and analysis of SK-OV-3 tumor progression in nude mice.

Inhibition of cell viability in SK-OV-3 Luccells following treatment with sera from BALB/c mice immunized with PBS (NoMS), scFv40 (scFv40MS), scFv69 (scFv69MS) or with HER2-Fc(HER2-FcMS). Cell number was proportional to the emitted luminescence and results were normalized to the fluorescence of untreated cells. Grey boxes represent the 25th and 75th percentiles with the medians as black lines; whiskers mark the smallest and largest non-outlier observations, and outliers are indicated by dots.

Ladjemi, M. Z., Chardes, T., Corgnac, S., Garambois, V., Morisseau, S., Robert, B., ... & Pelegrin, A. (2011). Vaccination with human anti-trastuzumab anti-idiotype scFv reverses HER2 immunological tolerance and induces tumor immunity in MMTV. f. huHER2 (Fo5) mice. Breast Cancer Research, 13(1), R17.

Figure 4 Tumor growth was evaluated by measuring the emitted luminescence once a week following injection of luciferin.

Bioluminescence imaging of nude mice xenografted with SK-OV-3-Luc tumor cells and treated with scFv69MS was performed at the end of the treatment (Day 27 after xenograft).

Ladjemi, M. Z., Chardes, T., Corgnac, S., Garambois, V., Morisseau, S., Robert, B., ... & Pelegrin, A. (2011). Vaccination with human anti-trastuzumab anti-idiotype scFv reverses HER2 immunological tolerance and induces tumor immunity in MMTV. f. huHER2 (Fo5) mice. Breast Cancer Research, 13(1), R17.