Chicken Anti-TRIM21 Recombinant Antibody (clone R2E5) (CAT#: FAMAB-0007JF)
Recombinant chicken monoclonal antibody specially binds to human TRIM21. This anti-TRIM21 antibody R2E5 can be potentially wildly used in the treatment of Sjögren's syndrome.
Specific Inquiry
Neut
Figure 1 Late MAV-1 antisera reduces viremia and protects T21.
Data are representative of at least 3 independent experiments.
Vaysburd, M., Watkinson, R. E., Cooper, H., Reed, M., O’Connell, K., Smith, J., ... & James, L. C. (2013). Intracellular antibody receptor TRIM21 prevents fatal viral infection. Proceedings of the National Academy of Sciences, 110(30), 12397-12401.
WB
Figure 2 TRIM21 NFκB signalling is constitutively silent despite monomeric RING K63 chain synthesis.
TRIM21 (T21) overexpression in 293Ts activates an NFκB-luciferase reporter.
Dickson, C., Fletcher, A. J., Vaysburd, M., Yang, J. C., Mallery, D. L., Zeng, J., ... & Cruickshank, J. (2018). Intracellular antibody signalling is regulated by phosphorylation of the Fc receptor TRIM21. Elife, 7, e32660.
Inhib
Figure 3 B-Box autoinhibition regulates antibody-dependent immune signalling by TRIM21.
Phosphomimetic mutation S80E potentiates transcriptional activation by TRIM21, while S80A inhibits activity.
Dickson, C., Fletcher, A. J., Vaysburd, M., Yang, J. C., Mallery, D. L., Zeng, J., ... & Cruickshank, J. (2018). Intracellular antibody signalling is regulated by phosphorylation of the Fc receptor TRIM21. Elife, 7, e32660.
IHC
Figure 4 TRIM21 expression is decreased in breast cancer.
TRIM21 expression in 169 paraffin-embedded samples was examined by IHC.
Zhou, W., Zhang, Y., Zhong, C., Hu, J., Hu, H., Zhou, D., & Cao, M. (2018). Decreased expression of TRIM21 indicates unfavorable outcome and promotes cell growth in breast cancer. Cancer management and research, 10, 3687.
FuncS
Figure 5 Kaplan–Meier overall survival curves for the patients with and without serum tripartite motif-containing 21 (TRIM21) antibodies (s-TRIM21-Abs).
P-value was calculated by log-rank test. The survival of s-TRIM21-Abs-negative patients was superior to that of s-TRIM21-Abs-positive patients.
Kuboshima, M., Shimada, H., Liu, T. L., Nomura, F., Takiguchi, M., Hiwasa, T., & Ochiai, T. (2006). Presence of serum tripartite motif‐containing 21 antibodies in patients with esophageal squamous cell carcinoma. Cancer science, 97(5), 380-386.
Specifications
- Immunogen
- Antigen mixture 1 (annexin XI, centromere protein B, DNA topoisomerase I, histidyl tRNA synthetase, Ro60, Rpp38 and U1A) and mixture 2 (a-fodrin, human Pop1 protein, heat shock protein B3, Ro52, Rpp30, Rrp4 and Rrp42)
- Host Species
- Chicken
- Derivation
- Chicken phage display scFv library
- Specificity
- Human TRIM21
- Species Reactivity
- Human
- Clone
- R2E5
- Applications
- WB, Neut, Inhib, FuncS, IHC
- Related Disease
- Sjögren's syndrome
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The TRIM21 antibody has been reported in applications of Western Blot, Neutralization, Inhibition, Immunohistochemistry, Functional Assay.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1189z | Mouse Anti-TRIM21 Recombinant Antibody (clone 20C4) | WB, ELISA | Mouse IgG1 |
| VS3-QX1165 | Mouse Anti-TRIM21 Recombinant Antibody (clone 5B9-B9-H8) | WB | Mouse IgG1 |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0007JF-F(E) | Chicken Anti-TRIM21 Recombinant Antibody (clone R2E5); Fab Fragment | IB, WB, ELISA, FuncS | Chicken Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0007JF-S(P) | Chicken Anti-TRIM21 Recombinant Antibody (clone R2E5); scFv Fragment | IB, WB, ELISA, FuncS | Chicken scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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