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Rabbit Anti-ZEB1 Recombinant Antibody (clone H-102) (CAT#: VS3-CJ921)

This product is a rabbit antibody that recognizes human, mouse, and rat ZEB1.


See more Engineered Antibody products for ZEB1
Specific Inquiry
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  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27524/if_selected.jpg
Figure 2 IHC staining of human smooth muscle
Figure 2 IHC staining of human smooth muscle
(Immunohistochemical staining of human smooth muscle shows strong nuclear positivity in smooth muscle cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79943/170691_B_6_7_selected.jpg
Figure 3 IF staining of human cell line U-251 MG
Figure 3 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27524/218_C10_1_red_green.jpg
Figure 4 IF staining of human cell line A549
Figure 4 IF staining of human cell line A549
(Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79943/2000_B6_1_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Figure 5 Cerebral cortex
(Endothelial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58686/125920_B_9_5.jpg
Figure 6 Colon
Figure 6 Colon
(Endothelial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58686/125920_A_9_3.jpg
Figure 7 Kidney
Figure 7 Kidney
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58686/125920_A_8_5.jpg
Figure 8 Testis
Figure 8 Testis
(Peritubular cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58686/125920_A_6_6.jpg
Figure 9 Smooth muscle
Figure 9 Smooth muscle
(Smooth muscle cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58686/125920_B_6_7.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, TIME)
Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, TIME)
(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000148516-ZEB1
Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, TIME)
Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, TIME)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000148516-ZEB1

Specifications

  • Immunogen
  • Amino acids 39-140 of ZEB1 of human origin
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse, Rat ZEB1
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • H-102
  • Applications
  • WB, IF, IP
  • Conjugate
  • Unconjugated

Product Property

  • Purification
  • Protein A affinity purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Format
  • Liquid
  • Buffer
  • 40% Glycerol, 1% BSA, TBS, pH7.4.
  • Preservative
  • 0.05% Sodium Azide
  • Storage
  • Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been tested for use in Western Blot, Immunofluorescence, Immunoprecipitation.

Target

  • Alternative Names
  • BZP; TCF8; AREB6; FECD6; NIL2A; PPCD3; ZFHEP; ZFHX1A; DELTAEF1
  • Sequence Similarities
  • Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.
  • Cellular Localization
  • Nucleus
  • Function
  • Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). Promotes tumorigenicity by repressing stemness-inhibiting microRNAs.

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "ZEB1"

Recombinant Antibody

CAT Product Name Application Type
DrMAB-693 Human Anti-ZEB1 Recombinant Antibody (clone U20) IP, IF, ELISA Human IgG
MOB-0682MZ Recombinant Mouse Anti-Human ZEB1 Antibody (clone 527B8I20) WB Mouse antibody
MOB-120LC Mouse Anti-ZEB1 Recombinant Antibody (clone 2A8A6) WB, FC Mouse IgG
VS3-XY1617 Mouse Anti-ZEB1 Recombinant Antibody (clone 2A8H3) ELISA, WB, IHC, FC, ICC Mouse IgG1
VS3-XY3543 Rabbit Anti-ZEB1 Recombinant Antibody (clone R01-5A7) WB, IHC-P, ICC, IF, FC Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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