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Mouse Anti-BACE1 Recombinant Antibody (clone 3C1C3) (CAT#: VS3-XY114)

Clone 3C1C3 is a mouse antibody, which reacts with human BACE1 protein. This antibody is suitable for BACE1 detection by ELISA, WB, ICC, FC assays.


See more Engineered Antibody products for BACE1
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16358/952_G12_1_selected.jpg
Figure 2 IHC staining of human pancreas
Figure 2 IHC staining of human pancreas
(Immunohistochemical staining of human pancreas shows cytoplasmic positivity.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16358/35706_A_1_3_selected.jpg
Figure 3 IF staining of human cell line A-431
Figure 3 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16358/947_G12_1_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Figure 4 Cerebral cortex
(Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16358/35706_B_7_5.jpg
Figure 5 Hippocampus
Figure 5 Hippocampus
(Glial cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16358/35706_B_8_6.jpg
Figure 6 Colon
Figure 6 Colon
(Glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16358/35706_A_7_3.jpg
Figure 7 Liver
Figure 7 Liver
(Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16358/35706_A_9_4.jpg
Figure 8 Kidney
Figure 8 Kidney
(Cells in tubules
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16358/35706_A_9_5.jpg
Figure 9 Testis
Figure 9 Testis
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining:Medium
Intensity: Moderate
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16358/35706_A_5_6.jpg
Figure 10 Lymph node
Figure 10 Lymph node
(Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16358/35706_A_7_8.jpg
RNA Expression
Figure 11 RNA cell line category: Cell line enhanced (fHDF/TERT166)
Figure 11 RNA cell line category: Cell line enhanced (fHDF/TERT166)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000186318-BACE1

Specifications

  • Immunogen
  • Recombinant fragment of human BACE1 (a.a. 112-324) expressed in E. coli.
  • Host Species
  • Mouse
  • Type
  • Mouse IgG1
  • Specificity
  • Human BACE1
  • Species Reactivity
  • Human
  • Clone
  • 3C1C3
  • Applications
  • Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry, Immunocytochemistry

Product Property

  • Purification
  • Affinity purified
  • Format
  • Liquid
  • Buffer
  • PBS
  • Preservative
  • 0.05% sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000), Flow Cytometry (1/200-1/400) and Immunocytochemistry (1/200-1/1000).

Target

  • Alternative Names
  • Beta-Secretase 1; Membrane-Associated Aspartic Protease 2; Beta-Site APP Cleaving Enzyme 1; Beta-Site APP-Cleaving Enzyme; Aspartyl Protease 2; EC 3.4.23.46; Memapsin-2; Asp 2; BACE; ASP2

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "BACE1"

Single-domain Antibody

CAT Product Name Application Type
NAB-145-VHH Recombinant Anti-Human BACE1 VHH Single Domain Antibody IHC, IP, FC, Neut, FUNC Llama VHH
PNBL-032 Recombinant Anti-Human BACE1 VHH Single Domain Antibody (XA4813) WB, ELISA, FuncS Llama VHH
HPAB-0354-YC-VHH Recombinant Dromedary Anti-BACE1 Single Domain Antibody (B9) ELISA, WB, Inhib, Activ Dromedary VHH

Recombinant Antibody

CAT Product Name Application Type
MOB-2939z Mouse Anti-BACE1 Recombinant Antibody (clone 18H11) WB, ELISA, FC, ICC Mouse IgG1
PABL-408 Human Anti-BACE1 Recombinant Antibody (PABL-408) ELISA, Block, Neut Human IgG
HPAB-0285-YC Mouse Anti-BACE1 Recombinant Antibody (HPAB-0285-YC) ELISA, FuncS Mouse IgG
HPAB-0672-CN Mouse Anti-BACE1 Recombinant Antibody (clone M2-1003) ELISA, FC, IHC, Neut Mouse IgG
HPAB-0673-CN Mouse Anti-BACE1 Recombinant Antibody (clone M2-1000) ELISA, FC, IHC, Neut Mouse IgG

Fab Fragment Antibody

CAT Product Name Application Type
PFBL-405 Human Anti-BACE1 Recombinant Antibody; Fab Fragment (PFBL-405) ELISA, Block, Neut Human Fab
HPAB-0285-YC-F(E) Mouse Anti-BACE1 Recombinant Antibody; Fab Fragment (HPAB-0285-YC-F(E)) ELISA, FuncS Mouse Fab
HPAB-0672-CN-F(E) Mouse Anti-BACE1 Recombinant Antibody (clone M2-1003); Fab Fragment ELISA, FC, IHC, Neut Mouse Fab
HPAB-0673-CN-F(E) Mouse Anti-BACE1 Recombinant Antibody (clone M2-1000); Fab Fragment ELISA, FC, IHC, Neut Mouse Fab

scFv Fragment Antibody

CAT Product Name Application Type
PSBL-405 Human Anti-BACE1 Recombinant Antibody; scFv Fragment (PSBL-405) ELISA, Block Human scFv
HPAB-0285-YC-S(P) Mouse Anti-BACE1 Recombinant Antibody; scFv Fragment (HPAB-0285-YC-S(P)) ELISA, FuncS Mouse scFv
HPAB-0672-CN-S(P) Mouse Anti-BACE1 Recombinant Antibody (clone M2-1003); scFv Fragment ELISA, FC, IHC, Neut Mouse scFv
HPAB-0673-CN-S(P) Mouse Anti-BACE1 Recombinant Antibody (clone M2-1000); scFv Fragment ELISA, FC, IHC, Neut Mouse scFv

Mouse Antibody

CAT Product Name Application Type
TAB-1156CL Mouse Anti-BACE1 Recombinant Antibody (TAB-1156CL) WB, IP, Inhib Mouse IgG
TAB-1153CL-S(P) Mouse Anti-BACE1 Recombinant Antibody; scFv Fragment (TAB-1153CL-S(P)) ELISA Mouse scFv
TAB-1154CL-S(P) Mouse Anti-BACE1 Recombinant Antibody; scFv Fragment (TAB-1154CL-S(P)) WB, ELISA Mouse scFv
TAB-1155CL-S(P) Mouse Anti-BACE1 Recombinant Antibody; scFv Fragment (TAB-1155CL-S(P)) ELISA, Inhib, FuncS Mouse scFv
TAB-1156CL-S(P) Mouse Anti-BACE1 Recombinant Antibody; scFv Fragment (TAB-1156CL-S(P)) ELISA, Inhib, FuncS Mouse scFv

Neutralizing Antibody

CAT Product Name Application Type
NEUT-036CQ Mouse Anti-BACE1 Recombinant Antibody (clone CBL278) ELISA, IP, Neut Mouse IgG1
NEUT-037CQ Mouse Anti-BACE1 Recombinant Antibody (clone CBL696) IP, Neut Mouse IgG1

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0315 Hi-Affi™ Rabbit Anti-BACE1 Recombinant Antibody (clone DS315AB) WB, IP Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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