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Mouse Anti-CBX1 Recombinant Antibody (clone 4D10A6) (CAT#: VS3-XY170)

Clone 4D10A6 is a mouse antibody, which reacts with human CBX1 protein. This antibody is suitable for CBX1 detection by ELISA, WB, IHC, FC, ICC assays.


See more Engineered Antibody products for CBX1
Specific Inquiry
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  • Gene Expression
  • Datasheet
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  • COA
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12265/610_F6_2_selected.jpg
Figure 2 IHC staining of human cerebellum
Figure 2 IHC staining of human cerebellum
(Immunohistochemical staining of human cerebellum shows strong nuclear positivity in cerebellar tissue.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12265/ihc_selected.jpg
Figure 3 IF staining of human cell line A-431
Figure 3 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12265/617_F6_1_red_green.jpg
Figure 4 IF staining of human cell line U-2 OS
Figure 4 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12265/613_F6_1_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Figure 5 Cerebral cortex
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12265/27516_B_8_5.jpg
Figure 6 Colon
Figure 6 Colon
(Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12265/27516_A_9_3.jpg
Figure 7 Liver
Figure 7 Liver
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12265/27516_A_7_4.jpg
Figure 8 Kidney
Figure 8 Kidney
(Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12265/27516_A_7_5.jpg
Figure 9 Testis
Figure 9 Testis
(Pachytene spermatocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Peritubular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Preleptotene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining:High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12265/27516_A_6_6.jpg
Figure 10 Lymph node
Figure 10 Lymph node
(Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12265/27516_A_9_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Figure 11 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000108468-CBX1

Specifications

  • Immunogen
  • Recombinant fragment of human CBX1 (a.a. 1-185) expressed in E. coli.
  • Host Species
  • Mouse
  • Type
  • Mouse IgG1
  • Specificity
  • Human CBX1
  • Species Reactivity
  • Human
  • Clone
  • 4D10A6
  • Applications
  • Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry

Product Property

  • Purification
  • Affinity purified
  • Format
  • Liquid
  • Buffer
  • PBS
  • Preservative
  • 0.05% sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000), Immunohistochemistry (1/200-1/1000), Flow Cytometry (1/200-1/400) and Immunocytochemistry (1/200-1/1000).

Target

  • Alternative Names
  • CBX, HP1-BETA, HP1Hs-beta, HP1Hsbeta, M31, MOD1, p25beta

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "CBX1"

Fab Fragment Antibody

CAT Product Name Application Type
DrMAB-268 Human Anti-CBX1 Recombinant Antibody (clone D256); Fab Fragment IF, ELISA, IP-MS, ChIP Human Fab

Recombinant Antibody

CAT Product Name Application Type
MOB-0350CT Mouse Anti-CBX1 Recombinant Antibody (clone 6B4) ELISA, FC, IF, WB Mouse IgG1
ZG-0442J Mouse Anti-CBX1 Recombinant Antibody (ZG-0442J) WB Mouse IgG
VS3-CJ73 Mouse Anti-CBX1 Recombinant Antibody (VS3-CJ73) WB, ELISA Mouse IgG

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0494 Hi-Affi™ Rabbit Anti-CBX1 Recombinant Antibody (clone DS494AB) WB Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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