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  • Mouse Anti-CD74 Recombinant Antibody (clone 2D1B11)

Mouse Anti-CD74 Recombinant Antibody (clone 2D1B11) (CAT#: VS3-XY404)

Clone 2D1B11 is a mouse antibody, which reacts with human CD74 protein. This antibody is suitable for CD74 detection by ELISA, WB, FC assays.


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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/if_selected.jpg
Figure 2 IHC staining of human spleen
Figure 2 IHC staining of human spleen
(Immunohistochemical staining of human spleen shows strong granular cytoplasmic positivity.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10592/ihc_selected.jpg
Figure 3 IHC staining of human tonsil
Figure 3 IHC staining of human tonsil
(Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in germinal center cells and non-germinal center cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79994/170659_A_6_8_selected.jpg
Figure 4 IF staining of human cell line U-251 MG
Figure 4 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/203_F11_1_blue_red_green.jpg
Normal Tissue
Figure 5 Colon
Figure 5 Colon
(Mucosal lymphoid cells
Staining:High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/6593_A_7_3.jpg
Figure 6 Kidney
Figure 6 Kidney
(Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/6593_A_8_5.jpg
Figure 7 Lymph node
Figure 7 Lymph node
(Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/6593_A_8_8.jpg
Figure 8 Tonsil
Figure 8 Tonsil
(Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/6593_A_6_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (CAPAN-2, Daudi, HDLM-2, REH, RPMI-8226, U-266/70, U-698)
Figure 9 RNA cell line category: Cell line enhanced (CAPAN-2, Daudi, HDLM-2, REH, RPMI-8226, U-266/70, U-698)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000019582-CD74

Specifications

  • Immunogen
  • Recombinant fragment of human CD74 (a.a. 1-106) expressed in E. coli.
  • Host Species
  • Mouse
  • Type
  • Mouse IgG1
  • Specificity
  • Human CD74
  • Species Reactivity
  • Human
  • Clone
  • 2D1B11
  • Applications
  • Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry

Product Property

  • Purification
  • Affinity purified
  • Format
  • Liquid
  • Buffer
  • PBS
  • Preservative
  • 0.05% sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000) and Flow Cytometry (1/200-1/400).

Target

  • Alternative Names
  • II; p33; CLIP; DHLAG; HLADG; Ia-GAMMA

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "CD74"

Single-domain Antibody

CAT Product Name Application Type
NAB-466-VHH Recombinant Anti-Human CD74 VHH Single Domain Antibody FUNC, FA, IP, ChiP Llama VHH
TAB-0004CL-VHH Anti-Human CD74 VHH Single Domain Antibody (NbhCD74n49 (Nb_49)) ELISA, FC, Internalization, Inhib, FuncS VHH
TAB-0005CL-VHH Anti-Human CD74 VHH Single Domain Antibody (NbhCD74n95 (Nb_95)) ELISA, FC, Internalization, Inhib, FuncS VHH
TAB-0006CL-VHH Anti-Human CD74 VHH Single Domain Antibody (Nb_03) ELISA, FC, Internalization, Inhib, FuncS VHH
TAB-0007CL-VHH Anti-Human CD74 VHH Single Domain Antibody (Nb_04) ELISA, FC, Internalization, Inhib, FuncS VHH

Human Antibody

CAT Product Name Application Type
TAB-0569CL Human Anti-CD74 Recombinant Antibody (TAB-0569CL) IHC, WB Human IgG
TAB-0570CL Human Anti-CD74 Recombinant Antibody (TAB-0570CL) ELISA, FC Human IgG
TAB-0571CL Human Anti-CD74 Recombinant Antibody (TAB-0571CL) ELISA, FC Human IgG
TAB-0572CL Human Anti-CD74 Recombinant Antibody (TAB-0572CL) ELISA, FC Human IgG
TAB-0573CL Human Anti-CD74 Recombinant Antibody (TAB-0573CL) ELISA Human IgG

MHC Tetramer for Cancer

CAT Product Name Application Type
MHC-LC1596 APC-DRB*01:01/Human CD74 (KPVSKMRMATPLLMQALPM) MHC Tetramer FCM
MHC-LC1599 APC-DRB*01:01/Human CD74 (KMRMATPLLMQALPM) MHC Tetramer FCM
MHC-LC1713 APC-DRB*01:01/Human CD74 (MKMRMATPLLMQALPM) MHC Tetramer FCM
MHC-LC2820 PE-H2-IAb/Human CD74 (KPVSKMRMATPLLMQAL) MHC Tetramer FCM
MHC-LC2821 PE-H2-IAd/Human CD74 (KPVSKMRMATPLLMQAL) MHC Tetramer FCM

Blocking Antibody

CAT Product Name Application Type
NEUT-408CQ Mouse Anti-CD74 Recombinant Antibody (clone LN2) FC, IHC-Fr, IHC-P, ICC, IP, WB, Block Mouse IgG1, κ

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0579 Hi-Affi™ Rabbit Anti-CD74 Recombinant Antibody (clone DS579AB) ICC, IHC-P, IP, WB Rabbit IgG

scFv Fragment Antibody

CAT Product Name Application Type
HPAB-0388-FY-S(P) Human Anti-CD74 Recombinant Antibody; scFv Fragment (HPAB-0388-FY-S(P)) FC Human scFv

Fab Fragment Antibody

CAT Product Name Application Type
HPAB-0388-FY-F(E) Human Anti-CD74 Recombinant Antibody; Fab Fragment (HPAB-0388-FY-F(E)) FC Human Fab

Recombinant Antibody

CAT Product Name Application Type
ZG-0135J Mouse Anti-CD74 Recombinant Antibody (ZG-0135J) IHC-P, IF Mouse IgG
ZG-0136J Mouse Anti-CD74 Recombinant Antibody (ZG-0136J) IHC-P, IF Mouse IgG
ZG-0722F Mouse Anti-CD74 Recombinant Antibody (clone 423L) FC Mouse IgG2a
VS4-WK92 Human Anti-CD74 Recombinant Antibody (clone 6B4) ELISA Human IgG1
VS-0723-WK70 Human Anti-CD74 Recombinant Antibody (VS-0723-WK70) FC Chimeric (rabbit/human) IgG1

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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