Mouse Anti-HSPA4 Recombinant Antibody (clone 5F2D10) (CAT#: VS3-XY830)
Clone 5F2D10 is a mouse antibody, which reacts with human HSPA4 protein. This antibody is suitable for HSPA4 detection by ELISA, WB, IHC, ICC assays.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10023/100_C10_1_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10023/100_C10_1_selected.jpg
Figure 2 IHC staining of human testis
(Immunohistochemical staining of human testis shows nuclear and cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10023/24928_A_6_6_selected.jpg
(Immunohistochemical staining of human testis shows nuclear and cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10023/24928_A_6_6_selected.jpg
Figure 3 IHC staining of human prostate
(Immunohistochemical staining of human prostate shows strong cytoplasmic and nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25529/60475_A_1_5_selected.jpg
(Immunohistochemical staining of human prostate shows strong cytoplasmic and nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25529/60475_A_1_5_selected.jpg
Figure 4 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10023/101_C10_1_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10023/101_C10_1_red_green.jpg
Figure 5 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10023/82_C10_1_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10023/82_C10_1_red_green.jpg
Figure 6 Cerebral cortex
(Glial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10023/25173_B_7_5.jpg
(Glial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10023/25173_B_7_5.jpg
Figure 7 Colon
(Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10023/24928_A_7_3.jpg
(Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10023/24928_A_7_3.jpg
Figure 8 Testis
(Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10023/24928_A_4_6.jpg
(Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10023/24928_A_4_6.jpg
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170606-HSPA4
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170606-HSPA4
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170606-HSPA4
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170606-HSPA4
Specifications
- Immunogen
- Recombinant fragment of human HSP70 expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human HSPA4
- Species Reactivity
- Human
- Clone
- 5F2D10
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunocytochemistry
Product Property
- Format
- Liquid
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000), Immunohistochemistry (1/200-1/1000) and Immunocytochemistry (1/200-1/1000).
Target
- Alternative Names
- Heat Shock Protein Family A (Hsp70) Member 4; Heat Shock 70-Related Protein APG-2; Heat Shock 70kDa Protein 4; Heat Shock 70kD Protein 4; Hsp70 RY; Hsp70RY; Epididymis Secretory Sperm Binding Protein Li 5a; Heat Shock 70 KDa Protein 4; Heat Shock Protein, 110 KDa
- Gene ID
- 3308
- UniProt ID
- P34932
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1694 | Rabbit Anti-HSPA4 Recombinant Antibody (clone DS1694AB) | WB, IP, ICC, ELISA, IHC | Rabbit IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0787-CN | Rabbit Anti-HSPA4 Recombinant Antibody (clone CBACN-296) | WB, IF, IHC, FC | Rabbit IgG |
| HPAB-A1209-YJ | Human Anti-HSPA4 Recombinant Antibody (clone G6A) | ELISA, IF | Human IgG |
| ZG-0455J | Mouse Anti-HSPA4 Recombinant Antibody (ZG-0455J) | WB, IHC, IF, ELISA | Mouse IgG |
| VS3-CJ889 | Rabbit Anti-HSPA4 Recombinant Antibody (VS3-CJ889) | WB, ICC, IF, IHC, FC | Rabbit IgG |
| VS4-WK231 | Mouse Anti-HSPA4 Recombinant Antibody (VS4-WK231) | ELISA, WB | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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