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Mouse Anti-MDM2 Recombinant Antibody (clone 3G2) (CAT#: VS3-XY1056)

Clone 3G2 is a mouse antibody, which reacts with MDM2 protein. This antibody is suitable for MDM2 detection by WB, IP, IF, IHC assays.


See more Engineered Antibody products for MDM2
Specific Inquiry
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  • Gene Expression
  • Datasheet
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  • COA
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16303/if_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/79977/1999_F7_3_selected.jpg
Figure 3 IHC staining of human cerebellum
Figure 3 IHC staining of human cerebellum
(Immunohistochemical staining of human cerebellum shows strong nuclear positivity.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/86/ihc_selected.jpg
Figure 4 IHC staining of human ovary
Figure 4 IHC staining of human ovary
(Immunohistochemical staining of human ovary shows strong nuclear positivity in ovarian stroma cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16303/37938_A_6_7_selected.jpg
Figure 5 IHC staining of human placenta
Figure 5 IHC staining of human placenta
(Immunohistochemical staining of human placenta shows strong nuclear and cytoplasmic positivity in trophoblastic cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/79977/170698_A_2_7_selected.jpg
Normal Tissue
Figure 6 Cerebral cortex
Figure 6 Cerebral cortex
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/86/33293_B_8_5.jpg
Figure 7 Colon
Figure 7 Colon
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/86/33293_A_9_3.jpg
Figure 8 Liver
Figure 8 Liver
(Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/86/33293_A_8_4.jpg
Figure 9 Pancreas
Figure 9 Pancreas
(Exocrine glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Pancreatic endocrine cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/86/33293_A_2_3.jpg
Figure 10 Kidney
Figure 10 Kidney
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/86/33293_A_8_5.jpg
Figure 11 Testis
Figure 11 Testis
(Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Peritubular cells
Staining: High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/86/33293_A_5_6.jpg
Figure 12 Lymph node
Figure 12 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/86/33293_A_9_8.jpg
RNA Expression
Figure 13 RNA cell line category: Cell line enhanced (HBEC3-KT, U-698)
Figure 13 RNA cell line category: Cell line enhanced (HBEC3-KT, U-698)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000135679-MDM2

Specifications

  • Immunogen
  • Amino acids 154-167 of MDM2 of human origin.
  • Host Species
  • Mouse
  • Type
  • Mouse IgG1
  • Specificity
  • MDM2
  • Species Reactivity
  • Human
  • Clone
  • 3G2
  • Applications
  • Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation

Product Property

  • Purification
  • Protein A affinity purified
  • Format
  • Liquid
  • Buffer
  • PBS
  • Preservative
  • 0.05% sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • MDM2 Proto-Oncogene; MDM2 Proto-Oncogene, E3 Ubiquitin Protein Ligase; Oncoprotein Mdm2; Hdm2; Mdm2, Transformed 3T3 Cell Double Minute 2, P53 Binding Protein (Mouse); Mdm2, Transformed 3T3 Cell Double Minute 2, P53 Binding Protein; Mouse Double Minute 2, Human Homolog Of; P53-Binding Protein; Double Minute 2, Human Homolog Of; P53-Binding Protein; Mdm2, P53 E3 Ubiquitin Protein Ligase Homolog; MDM2 Oncogene, E3 Ubiquitin Protein Ligase

Related Resources

  • Related Diseases
  • Related Signaling Pathways

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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Fab Fragment Antibody

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MHH-648-F(E) Recombinant Human Anti-human MDM2 Antibody Fab Fragment ELISA, WB, FC, FuncS Fab

scFv Fragment Antibody

CAT Product Name Application Type
MOB-648-S(P) Recombinant Anti-human MDM2 Antibody scFv Fragment ELISA, WB, FuncS scFv
MHH-648-S(P) Recombinant Human Anti-human MDM2 Antibody scFv Fragment ELISA, WB, FC, FuncS scFv

Recombinant Antibody

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MHH-648 Recombinant Human Anti-human MDM2 Antibody IP, RIA, FuncS IgG
ZG-0396U Rabbit Anti-MDM2 Recombinant Antibody (clone E22-L) IHC-P Rabbit IgG
VS3-FY917 Recombinant Mouse Anti-MDM2 Antibody (clone 3C1E3) ELISA, IHC, FC Mouse IgG2b
VS3-FY918 Recombinant Mouse Anti-MDM2 Antibody (clone 4A8D12) ELISA, FC Mouse IgG1
VS3-FY919 Recombinant Rabbit Anti-MDM2 (phospho Ser166) Antibody (clone R08-8C7) WB, IHC-P Rabbit IgG

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0353MZ Chicken Anti-MDM2 Polyclonal IgY WB Chicken antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-4642 Hi-Affi™ Recombinant Rabbit Anti-MDM2 Monoclonal Antibody (TH155DS) WB, IF, ICC IgG

MHC Tetramer for Cancer

CAT Product Name Application Type
MHC-CN1420 PE-A*02:01/Human MDM2 (LLGDLFGV) MHC Tetramer FCM
MHC-YF287 A*02:01/Human MDM2 (LLGDLFGV) MHC Monomer MHC Multimer

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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