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Mouse Anti-RING1 Recombinant Antibody (clone 8E8A2) (CAT#: VS3-XY1374)

Clone 8E8A2 is a mouse antibody, which reacts with human RING1 protein. This antibody is suitable for RING1 detection by ELISA, WB, ICC assays.


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Subcellular Location
Figure 1 IF staining of human cell line A-431
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8701/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Figure 2 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8701/23720_B_9_5.jpg
Figure 3 Nasopharynx
Figure 3 Nasopharynx
(Respiratory epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8701/23720_B_7_2.jpg
Figure 4 Colon
Figure 4 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8701/23720_A_9_3.jpg
Figure 5 Kidney
Figure 5 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8701/23720_A_7_5.jpg
Figure 6 Testis
Figure 6 Testis
(Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Peritubular cells Staining: Medium Intensity: Moderate Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8701/23720_A_4_6.jpg
Figure 7 Lymph node
Figure 7 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8701/23720_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000204227-RING1

Specifications

  • Immunogen
  • Recombinant fragment of human RING1 (a.a. 79-263) expressed in E. coli.
  • Host Species
  • Mouse
  • Type
  • Mouse IgG1
  • Specificity
  • Human RING1
  • Species Reactivity
  • Human
  • Clone
  • 8E8A2
  • Applications
  • Western Blot, Immunocytochemistry, Enzyme-linked Immunosorbent Assay

Product Property

  • Purification
  • Affinity purified
  • Format
  • Liquid
  • Buffer
  • PBS
  • Preservative
  • 0.05% sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody is recommended for Western Blot (1/500-1/2000), Immunocytochemistry (1/200-1/1000) and Enzyme-linked Immunosorbent Assay (1/10000).

Target

  • Alternative Names
  • Ring Finger Protein 1; RING-Type E3 Ubiquitin Transferase RING1; Really Interesting New Gene 1 Protein; Polycomb Complex Protein RING1; RING Finger Protein 1; RNF1

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "RING1"

Recombinant Antibody

CAT Product Name Application Type
MOB-2624z Mouse Anti-RING1 Recombinant Antibody (clone 27F9) WB, ELISA, FC, ICC, IF, IHC Mouse IgG1
MOB-1653MZ Recombinant Mouse Anti-Human RING1 Antibody WB Mouse antibody
VS3-QX973 Mouse Anti-RING1 Recombinant Antibody (clone 7D12-G1-B7) WB, IHC, ICC, FC, ELISA Mouse IgG1
VS3-WK82 Mouse Anti-RING1 Recombinant Antibody (clone F4-C12) WB, ELISA Mouse IgG1

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-3073 Hi-Affi™ Recombinant Rabbit Anti-RING1 Monoclonal Antibody (DS3073AB) IHC-P, WB, ICC/IF IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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