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Recombinant Rabbit Anti-ALYREF Antibody (clone R01-2A4) (CAT#: VS3-FY73)

This product is a recombinant rabbit antibody that recognizes human, mouse and rat ALYREF protein. It can detect a band of approximately 27 kDa (observedd molecular weight: 27 kDa).


See more Engineered Antibody products for ALYREF
Specific Inquiry
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  • Gene Expression
  • Datasheet
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19799/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human gallbladder
Figure 2 IHC staining of human gallbladder
(Immunohistochemical staining of human gallbladder shows nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16281/38406_A_6_4_selected.jpg
Figure 3 Lymph node
Figure 3 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16281/38406_A_7_8.jpg
Figure 4 Cerebral cortex
Figure 4 Cerebral cortex
(Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16281/38406_B_7_5.jpg
Figure 5 Colon
Figure 5 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16281/38406_A_7_3.jpg
Figure 6 Liver
Figure 6 Liver
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16281/38406_A_9_4.jpg
Figure 7 Kidney
Figure 7 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16281/38406_A_8_5.jpg
Figure 8 Testis
Figure 8 Testis
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16281/38406_A_6_6.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000183684-ALYREF

Specifications

  • Immunogen
  • A synthetic peptide of human Aly
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse and Rat ALYREF
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • R01-2A4
  • Applications
  • Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
  • Conjugate
  • Unconjugated
  • MW
  • Calculated MW: 27 kDa; Observed MW: 27 kDa

Product Property

  • Purification
  • Affinity Purified
  • Purity
  • >95%
  • Format
  • Liquid
  • Buffer
  • 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
  • Preservative
  • 0.01% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunohistochemistry: 1/50-1/100
    Immunofluorescence: 1/50-1/200
    Immunoprecipitation: 1/20

Target

  • Alternative Names
  • Aly/REF Export Factor; Transcriptional Coactivator Aly/REF; BZIP-Enhancing Factor BEF; Ally Of AML-1 And LEF-1; THO Complex 4; THOC4; Tho4

Recommended Products

Secondary Antibody

Isotype Control

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "ALYREF"

Recombinant Antibody

CAT Product Name Application Type
MOB-1890z Mouse Anti-ALYREF Recombinant Antibody (clone 11H8) WB, ELISA, ICC, IF, IP Mouse IgG1
VS1-YJ7 Mouse Anti-ALYREF Recombinant Antibody (clone 11G5) FC, IHC-P, ICC, ELISA Mouse IgG1

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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