Recombinant Mouse Anti-B. anthracis Anthrax LF Antibody (LF8) (CAT#: HPAB-0523-YJ)
This product is a recombinant mouse antibody that recognizes (both natural and recombinant) B. anthracis Anthrax LF. It may effectively neutralize anthrax lethal toxin and be used for the preparation of antibody medicine for the treatment of human anthrax infection.
Specific Inquiry
Neut
Figure 1 Neutralizing LF-specific MAbs bind sequential B-cell epitopes of LF.
Anti-LF MAbs 10G3, 9A11, and LF8 neutralize anthrax LeTx in vitro.
Nguyen, M. L., Crowe, S. R., Kurella, S., Teryzan, S., Cao, B., Ballard, J. D., ... & Farris, A. D. (2009). Sequential B-cell epitopes of Bacillus anthracis lethal factor bind lethal toxin-neutralizing antibodies. Infection and immunity, 77(1), 162-169.
Inhib
Figure 2 Neutralizing LF-specific MAbs bind sequential B-cell epitopes of LF.
Linear peptides corresponding to MAbs 10G3, 9A11, and LF8 mediate measurable reductions in neutralization in vitro (striped bars). Inhibition of neutralization is compared to the results for the same MAb mixed with toxin and irrelevant peptide from ovalbumin (OVA) (open bars). All experiments were carried out in duplicate. Since duplicate values were nearly identical, error bars are not shown. The concentrations of MAbs used for peptide inhibition were 1.25 μg/ml for MAb 9A11 and 5 μg/ml for MAbs 10G3 and LF8.
Nguyen, M. L., Crowe, S. R., Kurella, S., Teryzan, S., Cao, B., Ballard, J. D., ... & Farris, A. D. (2009). Sequential B-cell epitopes of Bacillus anthracis lethal factor bind lethal toxin-neutralizing antibodies. Infection and immunity, 77(1), 162-169.
IP
Figure 3 Immunoprecipitation analysis of anti-LF mAbs.
LF was immunoprecipitated with purified anti-LF mAbs and detected by Western blot analysis
Zhao, P., Liang, X., Kalbfleisch, J., Koo, H. M., & Cao, B. (2003). Neutralizing monoclonal antibody against anthrax lethal factor inhibits intoxication in a mouse model. Human antibodies, 12(4), 129-135.
WB
Figure 4 Western blot analysis of anti-LF mAbs.
LF was immunoprecipitated with purified anti-LF mAbs and detected by Western blot analysis
Zhao, P., Liang, X., Kalbfleisch, J., Koo, H. M., & Cao, B. (2003). Neutralizing monoclonal antibody against anthrax lethal factor inhibits intoxication in a mouse model. Human antibodies, 12(4), 129-135.
ELISA
Figure 5 Competitive ELISA.
The binding activity of mAb LF8 to LF can be competed by phage clones 1 and 2 in a dose-dependent manner. The wild-type M13 was used as control.
Zhao, P., Liang, X., Kalbfleisch, J., Koo, H. M., & Cao, B. (2003). Neutralizing monoclonal antibody against anthrax lethal factor inhibits intoxication in a mouse model. Human antibodies, 12(4), 129-135.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- B. anthracis
- Species Reactivity
- Bacillus anthracis
- Clone
- LF8
- Applications
- Neut, Inhib, IP, WB, ELISA, Neut, FuncS
- Conjugate
- NONE
- Related Disease
- Anthrax infection
Product Property
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The Anthrax LF antibody has been reported in applications of Neutralization, Inhibition, Immunoprecipitation, Western Blot, Enzyme-linked Immunosorbent Assay, Neutralization, Functional Assay.
Target
- Alternative Names
- Anthrax lethal factor; LF
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0524-YJ | Recombinant Chimeric (Mouse/Human) Anti-B. anthracis Anthrax LF Antibody (HcLF8) | ELISA, Neut, FuncS | Chimeric (mouse/human) IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0523-YJ-S(P) | Recombinant Mouse Anti-B. anthracis Anthrax LF Antibody scFv Fragment (LF8) | ELISA, Neut, FuncS | Mouse scFv |
| HPAB-0524-YJ-S(P) | Recombinant Mouse Anti-B. anthracis Anthrax LF Antibody scFv Fragment (HcLF8) | ELISA, Neut, FuncS | Mouse scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0523-YJ-F(E) | Recombinant Mouse Anti-B. anthracis Anthrax LF Antibody Fab Fragment (LF8) | ELISA, Neut, FuncS | Mouse Fab |
| HPAB-0524-YJ-F(E) | Recombinant Chimeric (Mouse/Human) Anti-B. anthracis Anthrax LF Antibody Fab Fragment (HcLF8) | ELISA, Neut, FuncS | Chimeric (mouse/human) Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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