Recombinant Rabbit Anti-CANX Antibody (clone R07-2B1) (CAT#: VS3-FY186)

• Immunogen
• A synthetic peptide of human Calnexin

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human CANX

• Species Reactivity
• Human

• Clone
• R07-2B1

• Applications
• Western Blot

• Conjugate
• Unconjugated

• MW
• Calculated MW: 68 kDa; Observed MW: 90 kDa

• Purification
• Affinity Purified

• Purity
• >95%

• Format
• Liquid

• Buffer
• 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA

• Preservative
• 0.01% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000

• Alternative Names
• Calnexin; Major Histocompatibility Complex Class I Antigen-Binding Protein P88; IP90; P90; CNX

• Gene ID
• 821

• UniProt ID
• P27824

• Sequence Similarities
• Belongs to the calreticulin family.

• Cellular Localization
• Endoplasmic reticulum, Membrane

• Post Translation Modifications
• Phosphorylated at Ser-564 by MAPK3/ERK1. Phosphorylation by MAPK3/ERK1 increases its association with ribosomes (By similarity).
  Palmitoylation by DHHC6 leads to the preferential localization to the perinuclear rough ER. It mediates the association of calnexin with the ribosome-translocon complex (RTC) which is required for efficient folding of glycosylated proteins.
  Ubiquitinated, leading to proteasomal degradation. Probably ubiquitinated by ZNRF4.

• Protein Refseq
• NP_001019820.1; NP_001737.1

• Function
• Calcium-binding protein that interacts with newly synthesized monoglucosylated glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

• 2ND-057CQ
• Donkey Anti-Rabbit IgG (H&L) Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-060CQ
• Llama Anti-Rabbit IgG (H&L) Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody