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Recombinant Mouse Anti-CFB Capture Antibody (clone 1E4) (CAT#: VS3-WK1630)

This antibody is a mouse recombinant antibody against human CFB. The sandwich ELISA was established using matched antibody pair 1E4 and 5E1. The clone 1E4 can be used in ELISA analysis as a capture antibody at a dilution of 1/1000. It was developed by immunization of mouse with recombinant human CFB protein and then was produced as a recombinant antibody. The isotype of this product is mouse IgG2b.


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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to cell junctions.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/951/170_F11_1_selected.jpg
Figure 2 IF staining of human cell line ASC TERT1
Figure 2 IF staining of human cell line ASC TERT1
(Immunofluorescent staining of human cell line ASC TERT1 shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1817/1749_A9_28_cr5804bcaa17056_selected.jpg
Figure 3 IHC staining of human prostate
Figure 3 IHC staining of human prostate
(Immunohistochemical staining of human prostate shows strong cytoplasmic and membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1817/ihc_selected.jpg
Figure 4 IHC staining of human prostate
Figure 4 IHC staining of human prostate
(Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1832/ihc_selected.jpg
Normal Tissue
Figure 5 Cerebral cortex
Figure 5 Cerebral cortex
(Endothelial cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1832/6333_B_8_5.jpg
Figure 6 Colon
Figure 6 Colon
(Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1832/6333_A_7_3.jpg
Figure 7 Rectum
Figure 7 Rectum
(Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1832/6333_A_4_3.jpg
Figure 8 Liver
Figure 8 Liver
(Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1832/6333_A_8_4.jpg
Figure 9 Kidney
Figure 9 Kidney
(Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1832/6333_A_7_5.jpg
Figure 10 Lymph node
Figure 10 Lymph node
(Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1832/6333_A_8_8.jpg
RNA Expression
Figure 11 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, EFO-21, fHDF/TERT166, HSkMC, U-87 MG)
Figure 11 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, EFO-21, fHDF/TERT166, HSkMC, U-87 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000243649-CFB

Specifications

  • Immunogen
  • Recombinant human CFB protein
  • Host Species
  • Mouse
  • Type
  • Mouse IgG2b
  • Specificity
  • Human CFB
  • Species Reactivity
  • Human
  • Clone
  • 1E4
  • Applications
  • ELISA
  • Conjugate
  • Unconjugated

Product Property

  • Clonality
  • Monoclonal
  • Purification
  • Protein G affinity purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Aggregation
  • < 5%
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • 0.09% sodium azide
  • Storage
  • Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
  • Shipping
  • Ice packs

Applications

  • Application Notes
  • ELISA: 1/1000

Target

  • Alternative Names
  • Complement Factor B; B-Factor, Properdin; Properdin Factor B; C3/C5 Convertase; EC 3.4.21.47; PBF2; BFD; GBG; BF; Glycine-Rich Beta-Glycoprotein; Glycine-Rich Beta Glycoprotein
  • Long Name
  • Complement Factor B
  • Sequence Similarities
  • Belongs to the peptidase S1 family.
  • Cellular Localization
  • Secreted
  • Function
  • Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes.

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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Mouse Antibody

CAT Product Name Application Type
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TAB-060MZ-S(P) Mouse Anti-CFB Recombinant Antibody; scFv Fragment (TAB-060MZ-S(P)) ELISA Mouse scFv

Humanized Antibody

CAT Product Name Application Type
TAB-061MZ Human Anti-CFB Recombinant Antibody (TAB-061MZ) ELISA Humanized antibody
TAB-061MZ-S(P) Human Anti-CFB Recombinant Antibody; scFv Fragment (TAB-061MZ-S(P)) ELISA Humanized scFv
TAB-061MZ-F(E) Human Anti-CFB Recombinant Antibody; Fab Fragment (TAB-061MZ-F(E)) ELISA Humanized Fab

Recombinant Antibody

CAT Product Name Application Type
MOB-1339MZ Recombinant Mouse Anti-Human CFB Antibody (clone 7H22) ELISA, WB Mouse antibody
HPAB-0119-YJ Human Anti-CFB Recombinant Antibody (HPAB-0119-YJ) FuncS, Inhib, Block Humanized IgG
HPAB-M0511-YC Human Anti-CFB Recombinant Antibody (clone AAfBb) ELISA, Inhib Humanized IgG
HPAB-M0512-YC Mouse Anti-CFB Recombinant Antibody (clone AfBb) ELISA, Inhib Mouse IgG
HPAB-M0513-YC Mouse Anti-CFB Recombinant Antibody (HPAB-M0513-YC) ELISA, FC, Block Mouse IgG

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0673 Hi-Affi™ Rabbit Anti-CFB Recombinant Antibody (clone DS673AB) WB Rabbit IgG

scFv Fragment Antibody

CAT Product Name Application Type
HPAB-0145CQ-S(P) Human Anti-CFB Recombinant Antibody; scFv Fragment (HPAB-0145CQ-S(P)) ELISA, Inhib, FC Humanized scFv
HPAB-0146CQ-S(P) Mouse Anti-CFB Recombinant Antibody; scFv Fragment (HPAB-0146CQ-S(P)) ELISA, Inhib, FC Mouse scFv
HPAB-AP277-YC-S(P) Mouse Anti-CFB Recombinant Antibody; scFv Fragment (HPAB-AP277-YC-S(P)) ELISA, Inhib Mouse scFv
HPAB-AP278-YC-S(P) Human Anti-CFB Recombinant Antibody; scFv Fragment (HPAB-AP278-YC-S(P)) ELISA, Inhib Humanized scFv
HPAB-AP279-YC-S(P) Mouse Anti-CFB Recombinant Antibody; scFv Fragment (HPAB-AP279-YC-S(P)) ELISA, Inhib Mouse scFv

Fab Fragment Antibody

CAT Product Name Application Type
HPAB-M0511-YC-F(E) Human Anti-CFB Recombinant Antibody (clone AAfBb); Fab Fragment ELISA, Inhib Humanized Fab
HPAB-M0512-YC-F(E) Mouse Anti-CFB Recombinant Antibody (clone AfBb); Fab Fragment ELISA, Inhib Mouse Fab
HPAB-M0513-YC-F(E) Mouse Anti-CFB Recombinant Antibody; Fab Fragment (HPAB-M0513-YC-F(E)) ELISA, FC, Block Mouse Fab
HPAB-M0514-YC-F(E) Mouse Anti-CFB Recombinant Antibody; Fab Fragment (HPAB-M0514-YC-F(E)) ELISA, FC, Block Mouse Fab
HPAB-M0515-YC-F(E) Human Anti-CFB Recombinant Antibody; Fab Fragment (HPAB-M0515-YC-F(E)) ELISA, FC, Block Chimeric (Mouse/Human) Fab

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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