Recombinant Rabbit Anti-CTNNA1 Antibody (clone R07-7D5) (CAT#: VS3-FY422)

• Immunogen
• A synthetic peptide of human alpha 1 Catenin

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse and Rat CTNNA1

• Species Reactivity
• Human, Mouse, Rat

• Clone
• R07-7D5

• Applications
• Western Blot, Immunoprecipitation

• Conjugate
• Unconjugated

• MW
• Calculated MW: 100 kDa; Observed MW: 100 kDa

• Purification
• Affinity Purified

• Purity
• >95%

• Format
• Liquid

• Buffer
• 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA

• Preservative
• 0.01% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunoprecipitation: 1/20

• Alternative Names
• Catenin Alpha 1; Renal Carcinoma Antigen NY-REN-13; Alpha-E-Catenin; Catenin (Cadherin-Associated Protein), Alpha 1 (102kD); Catenin (Cadherin-Associated Protein), Alpha 1, 102kDa; Cadherin-Associated Protein

• Gene ID
• 1495

• UniProt ID
• P35221

• Sequence Similarities
• Belongs to the vinculin/alpha-catenin family.

• Cellular Localization
• Cell junction, Cell membrane, Cytoplasm, Cytoskeleton, Membrane

• Post Translation Modifications
• Sumoylated.
  Phosphorylation seems to contribute to the strength of cell-cell adhesion rather than to the basic capacity for cell-cell adhesion.

• Protein Refseq
• NP_001277236.1; NP_001277238.1; NP_001277239.1

• Function
• Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. Involved in the regulation of WWTR1/TAZ, YAP1 and TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation (By similarity).
  May play a crucial role in cell differentiation.

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

• 2ND-057CQ
• Donkey Anti-Rabbit IgG (H&L) Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-060CQ
• Llama Anti-Rabbit IgG (H&L) Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody