Recombinant Mouse Anti-EGF Detection Antibody (clone 5B3) (CAT#: VS3-WK1665)

• Immunogen
• Recombinant human EGF protein

• Host Species
• Mouse

• Type
• Mouse IgG2a

• Specificity
• Human EGF

• Species Reactivity
• Human

• Clone
• 5B3

• Applications
• ELISA, WB

• Conjugate
• Unconjugated

• Clonality
• Monoclonal

• Purification
• Protein G affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Aggregation
• < 5%

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• PBS

• Preservative
• 0.09% sodium azide

• Storage
• Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• WB: 1/500-1/1000
  ELISA: 1/1000

• Alternative Names
• Epidermal Growth Factor; Epidermal Growth Factor (Beta-Urogastrone); Pro-Epidermal Growth Factor; Beta-Urogastrone; HOMG4; URG

• Gene ID
• 1950

• UniProt ID
• P01133

• Long Name
• Epidermal Growth Factor

• Cellular Localization
• Membrane

• Post Translation Modifications
• O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).

• Protein Refseq
• NP_001171601.1

• Function
• EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro.

Capture antibody

• VS3-WK1664
• Recombinant Mouse Anti-EGF Capture Antibody (clone 6B8)

Isotype Control

• MOB-202CQ
• Mouse IgG2a, kappa Isotype Control Antibody

Secondary Antibody

• 2RD-A1564-YJ
• Rabbit Anti-Mouse IgG2a Secondary antibody (Clone RM219)