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ERCC2
Recombinant Rabbit Anti-ERCC2 Antibody (clone R08-2I3)

Recombinant Rabbit Anti-ERCC2 Antibody (clone R08-2I3) (CAT#: VS3-FY520)

This product is a recombinant rabbit antibody that recognizes human ERCC2 protein. It can detect a band of approximately 87 kDa (observedd molecular weight: 80 kDa).

See more Engineered Antibody products for ERCC2

Tested Data
Gene Expression
Datasheet
MSDS
COA

WB

Figure 1. Recombinant Rabbit Anti-ERCC2 Antibody (clone R08-2I3) in WB.

Figure 1. Recombinant Rabbit Anti-ERCC2 Antibody (clone R08-2I3) in WB.

Western blot analysis of XPD in Jurkat, Hela lysates using XPD Antibody.

Subcellular Location

Figure 1 IF staining of human cell line SiHa

Figure 1 IF staining of human cell line SiHa
(Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69266/1606_F6_1_selected.jpg

Normal Tissue

Figure 2 Cerebral cortex

Figure 2 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38057/113541_B_8_5.jpg

Figure 3 Colon

Figure 3 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38057/113541_A_9_3.jpg

Figure 4 Kidney

Figure 4 Kidney
(Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38057/113541_A_8_5.jpg

Figure 5 Testis

Figure 5 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38057/113541_A_6_6.jpg

Figure 6 Lymph node

Figure 6 Lymph node
(Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38057/113541_A_8_8.jpg

RNA Expression

Figure 7 RNA cell line category: Low cell line specificity

Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000104884-ERCC2

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
A synthetic peptide of human XPD

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human ERCC2

Species Reactivity
Human

Clone
R08-2I3

Applications
Western Blot, Immunocytochemistry, Immunofluorescence

Conjugate
Unconjugated

MW
Calculated MW: 87 kDa; Observed MW: 80 kDa

Product Property

Purification
Affinity Purified

Purity
>95%

Format
Liquid

Buffer
50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA

Preservative
0.01% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200

Target

Alternative Names
ERCC Excision Repair 2, TFIIH Core Complex Helicase Subunit; Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 2; TFIIH Basal Transcription Factor Complex Helicase XPB Subunit; TFIIH Basal Transcription Factor Complex 80 KDa Subunit; Xeroderma Pigmentosum Group D-Complementing Protein; Excision Repair Cross-Complementation Group 2; Xeroderma Pigmentosum Complementary Group D; Basic Transcription Factor 2 80 KDa Subunit; DNA Repair Protein Complementing XP-D Cells; DNA Excision Repair Protein ERCC-2; TFIIH 80 KDa Subunit; TFIIH P80; BTF2 P80; CXPD

Gene ID
2068

UniProt ID
P18074

Recommended Products

Secondary Antibody

Isotype Control

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "ERCC2"

Recombinant Antibody

CAT	Product Name	Application	Type
MOB-1793MZ	Recombinant Mouse Anti-Human ERCC2 Antibody	FC, WB	Mouse antibody

Rabbit Monoclonal Antibody

CAT	Product Name	Application	Type
MOR-1184	Hi-Affi™ Rabbit Anti-ERCC2 Recombinant Antibody (clone DS1184AB)	WB, IP	Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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