Recombinant Rabbit Anti-ETS1 Antibody (clone R04-8C7) (CAT#: VS3-FY524)
This product is a recombinant rabbit antibody that recognizes ETS1. This antibody has been reported for use in Western Blot. The antigen is human ETS1 synthetic Peptide. The clone R04-8C7 is specific for human, mouse ETS1.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line RH-30
(Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63230/1151_D9_1_selected.jpg
(Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63230/1151_D9_1_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2575/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2575/if_selected.jpg
Figure 3 High expression in lymph node.
(Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2575/6349_A_8_8.jpg
(Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2575/6349_A_8_8.jpg
Figure 4 Tonsil
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2575/6349_A_5_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2575/6349_A_5_8.jpg
Figure 5 RNA cell line category: Cell line enhanced (HEL, JURKAT, U-698)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000134954-ETS1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000134954-ETS1
Specifications
- Immunogen
- Human ETS1 Synthetic Peptide.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse ETS1
- Species Reactivity
- Human, Mouse
- Clone
- R04-8C7
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- Calculated MW: 50 kDa; Observed MW: 50 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- ETS Proto-Oncogene 1, Transcription Factor; Avian Erythroblastosis Virus E26 (V-Ets) Oncogene Homolog-1; V-Ets Avian Erythroblastosis Virus E26 Oncogene Homolog 1; EWSR2; P54; V-Ets Avian Erythroblastosis Virus E2 Oncogene Homolog 1
- Gene ID
- 2113
- UniProt ID
- P14921
- Sequence Similarities
- Belongs to the ETS family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Sumoylated on Lys-15 and Lys-227, preferentially with SUMO2; which inhibits transcriptional activity.
Ubiquitinated; which induces proteasomal degradation.
Phosphorylation at Ser-251, Ser-282 and Ser-285 by CaMK2/CaMKII in response to calcium signaling decreases affinity for DNA: an increasing number of phosphoserines causes DNA-binding to become progressively weaker.
- Protein Refseq
- NP_001137292.1; NP_001155894.1; NP_001317380.1
- Function
- Transcription factor (PubMed:10698492, PubMed:11909962).
Directly controls the expression of cytokine and chemokine genes in a wide variety of different cellular contexts (PubMed:20378371).
May control the differentiation, survival and proliferation of lymphoid cells (PubMed:20378371).
May also regulate angiogenesis through regulation of expression of genes controlling endothelial cell migration and invasion (PubMed:15247905, PubMed:15592518).
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "ETS1"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3991z | Mouse Anti-ETS1 Recombinant Antibody (clone 25F12) | ELISA, FC, IF, IHC, WB | Mouse IgG1 |
| MOB-2368MZ | Recombinant Mouse Anti-Human ETS1 Antibody (clone 2H22) | IHC-P, IP, WB | Mouse antibody |
| MRO-0557-CN | Rabbit Anti-ETS1 Recombinant Antibody (clone JM92-32) | WB, IHC, FC | Rabbit IgG |
| ZG-0324J | Mouse Anti-ETS1 Recombinant Antibody (ZG-0324J) | WB, IHC, ELISA | Mouse IgG |
| VS3-CJ658 | Mouse Anti-ETS1 Recombinant Antibody (VS3-CJ658) | WB, FC | Mouse IgG2b, κ |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1207 | Hi-Affi™ Rabbit Anti-ETS1 Recombinant Antibody (clone DS1207AB) | FC, WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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