Recombinant Rabbit Anti-GAP43 Antibody (clone R01-4F6) (CAT#: VS3-FY577)

• Immunogen
• Synthetic peptide of human GAP43.

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse, Rat GAP43

• Species Reactivity
• Human, Mouse, Rat

• Clone
• R01-4F6

• Applications
• Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

• Conjugate
• Unconjugated

• MW
• Calculated MW: 25 kDa; Observed MW: 46 kDa

• Purification
• Affinity Purified

• Purity
• >95% as determined by SDS-PAGE

• Buffer
• 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA

• Preservative
• 0.01% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunohistochemistry-Paraffin: 1/50-1/100
  Immunofluorescence: 1/50-1/200
  Immunoprecipitation: 1/20

• Alternative Names
• Growth Associated Protein 43; Axonal Membrane Protein GAP-43; Neural Phosphoprotein B-50; Neuron Growth-Associated Protein 43; Nerve Growth-Related Peptide GAP43; Calmodulin-Binding Protein P-57

• Gene ID
• 2596

• UniProt ID
• P17677

• Sequence Similarities
• Belongs to the neuromodulin family.

• Cellular Localization
• Cell membrane, Cell projection, Cytoplasm, Membrane, Synapse

• Post Translation Modifications
• Phosphorylated (By similarity).
  Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity (By similarity).
  Palmitoylated by ZDHHC3 (By similarity).
  Palmitoylation is regulated by ARF6 and is essential for plasma membrane association and axonal and dendritic filopodia induction (PubMed:14978216).
  Deacylated by LYPLA2 (PubMed:21152083).

• Protein Refseq
• NP_001123536.1; NP_002036.1

• Function
• This protein is associated with nerve growth. It is a major component of the motile 'growth cones' that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction.

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

• 2ND-057CQ
• Donkey Anti-Rabbit IgG (H&L) Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-060CQ
• Llama Anti-Rabbit IgG (H&L) Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody