Recombinant Rabbit Anti-GYS1 (phospho Ser641) Antibody (clone R03-3A1) (CAT#: VS3-FY634)

• Immunogen
• A synthetic phosphopeptide corresponding to residues surrounding Ser641 of human glycogen synthase 1/GYS1.

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse, Rat GYS1

• Species Reactivity
• Human, Mouse, Rat

• Clone
• R03-3A1

• Applications
• Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation

• Conjugate
• Unconjugated

• MW
• Calculated MW: 84 kDa; Observed MW: 84 kDa

• Purification
• Affinity Purified

• Purity
• >95% as determined by SDS-PAGE

• Buffer
• 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA

• Preservative
• 0.01% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunohistochemistry-Paraffin: 1/50-1/100
  Immunoprecipitation: 1/20

• Alternative Names
• Glycogen Synthase 1; Glycogen Synthase 1 (Muscle); EC 2.4.1.11; GYS; Glycogen [Starch] Synthase, Muscle; GSY

• Gene ID
• 2997

• UniProt ID
• P13807

• Sequence Similarities
• Belongs to the glycosyltransferase 3 family.

• Cellular Localization
• Cytoplasm, Cytosol

• Post Translation Modifications
• Phosphorylation at Ser-8 by AMPK inactivates the enzyme activity. Primed phosphorylation at Ser-657 (site 5) by CSNK2A1 and CSNK2A2 is required for inhibitory phosphorylation at Ser-641 (site 3a), Ser-645 (site 3b), Ser-649 (site 3c) and Ser-653 (site 4) by GSK3A an GSK3B (By similarity).
  Phosphorylated at Ser-641 by DYRK2, leading to inactivation (By similarity).
  Phosphorylated at Ser-641 by PASK, leading to inactivation; phosphorylation by PASK is inhibited by glycogen. Dephosphorylation at Ser-641 and Ser-645 by PP1 activates the enzyme.

• Protein Refseq
• NP_001155059.1; NP_002094.2

• Function
• Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

• 2ND-057CQ
• Donkey Anti-Rabbit IgG (H&L) Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-060CQ
• Llama Anti-Rabbit IgG (H&L) Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody