Recombinant Rabbit Anti-LAMP2 Antibody (clone R03-0B5) (CAT#: VS3-FY839)

• Immunogen
• Human LAMP2 recombinant protein.

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human LAMP2

• Species Reactivity
• Human

• Clone
• R03-0B5

• Applications
• Western Blot, Immunohistochemistry-Paraffin

• Conjugate
• Unconjugated

• MW
• Calculated MW: 45 kDa; Observed MW: 110 kDa

• Purification
• Affinity Purified

• Purity
• >95% as determined by SDS-PAGE

• Buffer
• 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA

• Preservative
• 0.01% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunohistochemistry-Paraffin: 1/50-1/100

• Alternative Names
• LAMP2; Lysosome-associated membrane glycoprotein 2; LAMP-2; Lysosome-associated membrane protein 2; CD107 antigen-like family member B; CD107b

• Gene ID
• 3920

• UniProt ID
• P13473

• Sequence Similarities
• Belongs to the LAMP family.

• Cellular Localization
• Cell membrane, Cytoplasmic vesicle, Endosome, Lysosome, Membrane

• Post Translation Modifications
• O- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans.

• Protein Refseq
• NP_001116078.1; NP_002285.1; NP_054701.1

• Function
• Plays an important role in chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins in response to various stresses and as part of the normal turnover of proteins with a long biological half-live (PubMed:8662539, PubMed:11082038, PubMed:18644871, PubMed:24880125, PubMed:27628032).
  Functions by binding target proteins, such as GAPDH and MLLT11, and targeting them for lysosomal degradation (PubMed:8662539, PubMed:11082038, PubMed:18644871, PubMed:24880125).
  Plays a role in lysosomal protein degradation in response to starvation (By similarity).
  Required for the fusion of autophagosomes with lysosomes during autophagy (PubMed:27628032).
  Cells that lack LAMP2 express normal levels of VAMP8, but fail to accumulate STX17 on autophagosomes, which is the most likely explanation for the lack of fusion between autophagosomes and lysosomes (PubMed:27628032).
  Required for normal degradation of the contents of autophagosomes (PubMed:27628032).
  Required for efficient MHCII-mediated presentation of exogenous antigens via its function in lysosomal protein degradation; antigenic peptides generated by proteases in the endosomal/lysosomal compartment are captured by nascent MHCII subunits (PubMed:20518820).
  Is not required for efficient MHCII-mediated presentation of endogenous antigens (PubMed:20518820).
  Isoform LAMP-2C
  Modulates chaperone-mediated autophagy. Decreases presentation of endogenous antigens by MHCII. Does not play a role in the presentation of exogenous and membrane-derived antigens by MHCII.
  (Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F.

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

• 2ND-057CQ
• Donkey Anti-Rabbit IgG (H&L) Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-060CQ
• Llama Anti-Rabbit IgG (H&L) Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody