Recombinant Rabbit Anti-LGALS3 Antibody (clone R01-5E1) (CAT#: VS3-FY846)

This product is a recombinant rabbit antibody that recognizes LGALS3. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R01-5E1 is specific for human LGALS3. The antigen is human galectin-3 recombinant protein.


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WB

Figure 1. Recombinant Rabbit Anti-LGALS3 Antibody (clone R01-5E1) in WB.

Figure 1. Recombinant Rabbit Anti-LGALS3 Antibody (clone R01-5E1) in WB.

Western Blot analysis of Galectin 3 in HeLa lysates using Galectin 3 Antibody.


Specifications

  • Immunogen
  • Human galectin-3 recombinant protein.
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human LGALS3
  • Species Reactivity
  • Human
  • Clone
  • R01-5E1
  • Applications
  • Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
  • Conjugate
  • Unconjugated
  • MW
  • Calculated MW: 26 kDa; Observed MW: 26 kDa

Product Property

  • Purification
  • Affinity Purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Buffer
  • 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
  • Preservative
  • 0.01% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunohistochemistry-Paraffin: 1/50-1/100
    Immunofluorescence: 1/50-1/200
    Immunoprecipitation: 1/20

Target

  • Alternative Names
  • L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2
  • Cellular Localization
  • Cytoplasm, Nucleus, Secreted, Spliceosome
  • Function
  • Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity).
    In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes.

Recommended Products

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "LGALS3"

Rabbit Monoclonal Antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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