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Recombinant Mouse Anti-LMNB1 Antibody (VS3-FY834) (CAT#: VS3-FY834)

This product is a recombinant mouse antibody that recognizes LMNB1. This antibody has been reported for use in Western Blot, Immunoprecipitation.


See more Engineered Antibody products for LMNB1
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line MCF7
Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to nuclear membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/50524/if_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5269/950_C1_1_selected.jpg
Figure 3 IF staining of human cell line U-2 OS
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/79757/1894_G3_4_selected.jpg
Figure 4 IHC staining of human colon
Figure 4 IHC staining of human colon
(Immunohistochemical staining of human colon shows nuclear membrane positivity in glandular and stroma cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/50524/ihc_selected.jpg
Figure 5 IHC staining of human stomach
Figure 5 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows strong nuclear membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5269/155655_A_3_1_selected.jpg
Figure 6 IHC staining of human esophagus
Figure 6 IHC staining of human esophagus
(Immunohistochemical staining of human esophagus shows strong nuclear positivity in squamous epithelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/79954/171056_B_6_1_selected.jpg
Normal Tissue
Figure 7 Cerebral cortex
Figure 7 Cerebral cortex
(Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/50524/140330_B_8_5.jpg
Figure 8 Kidney
Figure 8 Kidney
(Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/50524/140330_A_7_4.jpg
Figure 9 Testis
Figure 9 Testis
(Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/50524/140330_A_5_6.jpg
Figure 10 Lymph node
Figure 10 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/50524/140330_A_8_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Figure 11 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113368-LMNB1

Specifications

  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Human, Mouse, Rat LMNB1
  • Species Reactivity
  • Human, Mouse, Rat
  • Applications
  • Western Blot, Immunoprecipitation
  • Conjugate
  • Unconjugated
  • MW
  • 68 kDa

Product Property

  • Purification
  • Affinity Chromatography
  • Purity
  • >95% as determined by SDS-PAGE
  • Concentration
  • 1 mg/mL
  • Buffer
  • PBS, 50% glycerol, 0.5% BSA
  • Preservative
  • 0.02% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/2000-5000
    Immunoprecipitation: 1/200

Target

  • Alternative Names
  • LMNB1; LMN2; LMNB; Lamin-B1
  • Sequence Similarities
  • Belongs to the intermediate filament family.
  • Cellular Localization
  • Intermediate filament, Nucleus
  • Post Translation Modifications
  • B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
  • Function
  • Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.

Recommended Products

Secondary Antibody

Reconstitution Buffer

Recommended Dilution Buffer

Isotype Control

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "LMNB1"

Recombinant Antibody

CAT Product Name Application Type
MOB-1818z Mouse Anti-LMNB1 Recombinant Antibody (clone 19G6) ELISA, IHC, WB Mouse IgG2a, κ
MOB-0073F Mouse Anti-LMNB1 Recombinant Antibody (clone 7C11) WB, IHC-P, IF, IP Mouse IgG
MOB-0074F Mouse Anti-LMNB1 Recombinant Antibody (clone 7C11), AbFluor™ 350 WB, IHC-P, IF, IP Mouse IgG
MOB-0075F Mouse Anti-LMNB1 Recombinant Antibody (clone 7C11), AbFluor™ 405 WB, IHC-P, IF, IP Mouse IgG
ZG-0264C Mouse Anti-LMNB1 Recombinant Antibody (ZG-0264C) WB Mouse IgG

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-2068 Hi-Affi™ Recombinant Rabbit Anti-LMNB1 Monoclonal Antibody (DS2068AB) WB, IHC, IP IgG
MOR-0075-FY Rabbit Anti-LMNB1 Recombinant Antibody (clone AFY0046) ICC, IHC-P, WB Rabbit IgG

MHC Tetramer for Cancer

CAT Product Name Application Type
MHC-LC3725 PE-A*02:02/Human LMNB1 (ELRELNDRL) MHC Tetramer FCM
MHC-LC3726 PE-A*02:01/Human LMNB1 (ELRELNDRL) MHC Tetramer FCM
MHC-LC3727 PE-A*68:02/Human LMNB1 (ELRELNDRL) MHC Tetramer FCM
MHC-LC3728 PE-A*02:01/Human LMNB1 (RLAVYIDKV) MHC Tetramer FCM
MHC-LC3729 PE-A*68:02/Human LMNB1 (RLAVYIDKV) MHC Tetramer FCM

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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