Recombinant Rabbit Anti-MIF Capture Antibody (clone R02-1N7) (CAT#: VS3-WK1750)
This antibody is a rabbit recombinant antibody against human MIF. It was developed by immunization of rabbit with recombinant human MIF protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. The sandwich ELISA was established using matched antibody pair R02-1N7 and R07-1X2. The clone R02-1N7 can be used as a capture antibody in ELISA analysis to identify human MIF.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3868/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3868/if_selected.jpg
Figure 2 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5284/13362_A_8_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5284/13362_A_8_5.jpg
Figure 3 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5284/13362_A_5_6.jpg
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5284/13362_A_5_6.jpg
Figure 4 Prostate
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5284/13362_A_1_5.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5284/13362_A_1_5.jpg
Figure 5 RNA cell line category: Cell line enhanced (HEK93)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000240972-MIF
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000240972-MIF
Specifications
- Immunogen
- Recombinant human MIF protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human MIF
- Species Reactivity
- Human
- Clone
- R02-1N7
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01M PBS, pH 7.2
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- Macrophage Migration Inhibitory Factor; Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor); Glycosylation-Inhibiting Factor; Phenylpyruvate Tautomerase; L-Dopachrome Tautomerase; L-Dopachrome Isomerase
- Gene ID
- 4282
- UniProt ID
- P14174
- Long Name
- Macrophage Migration Inhibitory Factor
- Sequence Similarities
- Belongs to the MIF family.
- Cellular Localization
- Cytoplasm, Secreted
- Protein Refseq
- NP_002406.1
- Function
- Pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids.
Recommended Products
Detection antibody
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "MIF"
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-H41 | Anti-Human MIF Recombinant Antibody (Imalumab) | FuncS, IF, Neut, ELISA, FC, IP, IHC | IgG1 - kappa |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0806CL-S(P) | Mouse Anti-MIF Recombinant Antibody; scFv Fragment (TAB-0806CL-S(P)) | ELISA, FC | Mouse scFv |
| TAB-0807CL-S(P) | Mouse Anti-MIF Recombinant Antibody; scFv Fragment (TAB-0807CL-S(P)) | ELISA, FC | Mouse scFv |
| TAB-0808CL-S(P) | Mouse Anti-MIF Recombinant Antibody; scFv Fragment (TAB-0808CL-S(P)) | ELISA, FC | Mouse scFv |
| TAB-0809CL-S(P) | Mouse Anti-MIF Recombinant Antibody; scFv Fragment (TAB-0809CL-S(P)) | ELISA, FC | Mouse scFv |
| TAB-0810CL-S(P) | Mouse Anti-MIF Recombinant Antibody; scFv Fragment (TAB-0810CL-S(P)) | ELISA, FC | Mouse scFv |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1923MZ | Recombinant Mouse Anti-Human MIF Antibody (clone 2A10-4D3) | IHC-P, WB | Mouse antibody |
| HPAB-0104-YC | Human Anti-MIF Recombinant Antibody (HPAB-0104-YC) | ELISA, Activ | Human IgG4 |
| MRO-1000-CN | Recombinant Rabbit Anti-MIF Monoclonal Antibody (JM11-64) | WB, IP, FC | Rabbit IgG |
| HPAB-0331-WJ | Mouse Anti-MIF Recombinant Antibody (HPAB-0331-WJ) | ELISA | Mouse IgG |
| ZG-0468U | Rabbit Anti-MIF Recombinant Antibody (clone 7A9) | ELISA, WB | Rabbit IgG |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1741CQ | Recombinant Mouse Anti-MIF Antibody (10C3) | ELISA(Cap), WB, Neut | IgG2b, κ |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2234 | Hi-Affi™ Recombinant Rabbit Anti-MIF Monoclonal Antibody (DS2234AB) | WB, ICC/IF, IP, FC | IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0104-YC-S(P) | Human Anti-MIF Recombinant Antibody; scFv Fragment (HPAB-0104-YC-S(P)) | ELISA, Activ | Human scFv |
| HPAB-0331-WJ-S(P) | Mouse Anti-MIF Recombinant Antibody; scFv Fragment (HPAB-0331-WJ-S(P)) | ELISA | Mouse scFv |
| HPAB-0332-WJ-S(P) | Mouse Anti-MIF Recombinant Antibody; scFv Fragment (HPAB-0332-WJ-S(P)) | ELISA | Mouse scFv |
| HPAB-0333-WJ-S(P) | Mouse Anti-MIF Recombinant Antibody; scFv Fragment (HPAB-0333-WJ-S(P)) | ELISA | Mouse scFv |
| HPAB-1311-FY-F(E) | Human Anti-MIF Recombinant Antibody; Fab Fragment (HPAB-1311-FY-F(E)) | Inhib | Human Fab |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1328LY-F(E) | Human Anti-MIF Recombinant Antibody; Fab Fragment (HPAB-1328LY-F(E)) | Inhib, ELISA | Human Fab |
| HPAB-1311-FY-S(P) | Human Anti-MIF Recombinant Antibody; scFv Fragment (HPAB-1311-FY-S(P)) | Inhib | Human scFv |
| HPAB-1312-FY-S(P) | Human Anti-MIF Recombinant Antibody; scFv Fragment (HPAB-1312-FY-S(P)) | Inhib | Human scFv |
| HPAB-1313-FY-S(P) | Human Anti-MIF Recombinant Antibody; scFv Fragment (HPAB-1313-FY-S(P)) | Inhib | Human scFv |
| HPAB-1314-FY-S(P) | Human Anti-MIF Recombinant Antibody; scFv Fragment (HPAB-1314-FY-S(P)) | Inhib | Human scFv |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-H41 | Afuco™ Anti-MIF ADCC Recombinant Antibody (Imalumab), ADCC Enhanced | FuncS, IF, Neut, ELISA, FC, IP | ADCC enhanced antibody |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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