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  • Recombinant Mouse Anti-MKI67 Antibody (VS4-CJ36)

Recombinant Mouse Anti-MKI67 Antibody (VS4-CJ36) (CAT#: VS4-CJ36)

This mouse anti-MKI67 antibody is a product that recognizes human, mouse, rat MKI67. The immunogen of this antibody is a synthetic peptide corresponding to MKI67. The mAb has been tested for use in ELISA, Immunohistochemistry (1:100-1:200).


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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies & nucleoli rim.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1164/13_B8_2_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nucleoli rim.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58/952_H5_3_selected.jpg
Figure 3 IF staining of human cell line A-431
Figure 3 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nucleoli rim.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1164/30_B1_1_red_green.jpg
Normal Tissue
Figure 4 Colon
Figure 4 Colon
(Goblet cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58/155454_A_7_3.jpg
Figure 5 Testis
Figure 5 Testis
(Cells in seminiferous ducts
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58/155454_A_6_6.jpg
Figure 6 Skin
Figure 6 Skin
(Cells in basal layer
Staining: Medium
Intensity: Strong
Quantity: <25%
Cells in spinous layer
Staining: Medium
Intensity: Strong
Quantity: <25%
Hair follicles
Staining: High
Intensity: Strong
Quantity: 75%-25%
Lymphocytes
Staining: High
Intensity: Strong
Sebaceous glands
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58/155454_B_7_1.jpg
Figure 7 Lymph node
Figure 7 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58/155454_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000148773-MKI67
Figure 8 RNA cell line category: Low cell line specificity
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000148773-MKI67

Specifications

  • Immunogen
  • Synthetic Peptide
  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Human, Mouse, Rat MKI67
  • Species Reactivity
  • Human, Mouse, Rat
  • Applications
  • ELISA, IHC
  • Conjugate
  • Unconjugated

Product Property

  • Purification
  • Immunogen affinity chromatography
  • Purity
  • >95% as determined by SDS-PAGE
  • Format
  • Liquid
  • Buffer
  • 50% Glycerol, PBS, pH 7.4.
  • Preservative
  • 0.02% Sodium Azide
  • Storage
  • Store at -20°C or -80°C upon receipt. Avoid repeated freeze.

Applications

  • Application Notes
  • This antibody has been tested for use in ELISA, Immunohistochemistry (1:100-1:200).

Target

  • Alternative Names
  • KIA; MIB-; MIB-1; PPP1R105
  • Long Name
  • Marker of proliferation Ki-67
  • Cellular Localization
  • Chromosome, Nucleus
  • Post Translation Modifications
  • Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604).
    Hyperphosphorylated form does not bind DNA.
  • Function
  • Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226).
    Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226).
    Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226).
    Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551).
    Does not contribute to the internal structure of mitotic chromosomes (By similarity).
    May play a role in chromatin organization (PubMed:24867636).
    It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).

Recommended Products

Isotype Control

Secondary Antibody

Recommended Dilution Buffer

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "MKI67"

Recombinant Antibody

CAT Product Name Application Type
MOB-0003MZ Recombinant Mouse Anti-Human MKI67 Antibody (clone Li-68) ELISA Mouse antibody
MRO-0899-CN Recombinant Rabbit Anti-MKI67 Monoclonal Antibody (CBACN-337) WB, IHC, FC Rabbit IgG
MRO-1993-CN Rabbit Anti-MKI67 Polyclonal Antibody (MRO-1993-CN) WB, IF, IHC, FC Rabbit IgG
MRO-1994-CN Rabbit Anti-MKI67 Polyclonal Antibody (MRO-1994-CN) WB, IHC Rabbit IgG
HPAB-2357LY Mouse Anti-MKI67 Recombinant Antibody (clone 73043-24E8) IHC, ELISA, WB Mouse IgG

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-2237 Hi-Affi™ Recombinant Rabbit Anti-MKI67 Monoclonal Antibody (DS2237AB) ELISA, WB, IHC IgG
MOR-4638 Hi-Affi™ Recombinant Rabbit Anti-MKI67 Monoclonal Antibody (TH151DS) IF, ICC, FC, ChIP IgG
MOR-0054-FY Rabbit Anti-MKI67 Recombinant Antibody (clone AFY0025) ICC, IHC-P, WB Rabbit IgG

Fab Fragment Antibody

CAT Product Name Application Type
HPAB-2357LY-F(E) Mouse Anti-MKI67 Recombinant Antibody (clone 73043-24E8); Fab Fragment IHC, ELISA, WB Mouse Fab

scFv Fragment Antibody

CAT Product Name Application Type
HPAB-2357LY-S(P) Mouse Anti-MKI67 Recombinant Antibody (clone 73043-24E8); scfv Fragment IHC, ELISA, WB Mouse scfv

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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