Recombinant Rabbit Anti-MMP14 Antibody (clone R05-6H5) (CAT#: VS3-FY938)

• Immunogen
• Synthetic peptide of human MMP14.

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Hamster MMP14

• Species Reactivity
• Human, Hamster

• Clone
• R05-6H5

• Applications
• Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation

• Conjugate
• Unconjugated

• MW
• Calculated MW: 66 kDa; Observed MW: 66 kDa

• Purification
• Affinity Purified

• Purity
• >95% as determined by SDS-PAGE

• Buffer
• 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA

• Preservative
• 0.01% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunohistochemistry-Paraffin: 1/50-1/100
  Immunoprecipitation: 1/20

• Alternative Names
• Matrix Metallopeptidase 14; Matrix Metallopeptidase 14 (Membrane-Inserted); Membrane-Type-1 Matrix Metalloproteinase; Membrane Type 1 Metalloprotease; EC 3.4.24.80; MT-MMP 1; MT1-MMP; MMP-14; MMP-X1

• Gene ID
• 4323

• UniProt ID
• P50281

• Sequence Similarities
• Belongs to the peptidase M10A family.

• Cellular Localization
• Cytoplasm, Membrane

• Post Translation Modifications
• The precursor is cleaved by a furin endopeptidase.
  Tyrosine phosphorylated by PKDCC/VLK.

• Protein Refseq
• NP_004986.1

• Function
• Endopeptidase that degrades various components of the extracellular matrix such as collagen. Activates progelatinase A. Essential for pericellular collagenolysis and modeling of skeletal and extraskeletal connective tissues during development (By similarity).
  May be involved in actin cytoskeleton reorganization by cleaving PTK7 (PubMed:20837484).
  Acts as a positive regulator of cell growth and migration via activation of MMP15. Involved in the formation of the fibrovascular tissues in association with pro-MMP2 (PubMed:12714657).
  Cleaves ADGRB1 to release vasculostatin-40 which inhibits angiogenesis (PubMed:22330140).

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

• 2ND-057CQ
• Donkey Anti-Rabbit IgG (H&L) Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-060CQ
• Llama Anti-Rabbit IgG (H&L) Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody