Recombinant Mouse Anti-MYOD1 Antibody (clone 3A10-1C10-10D5) (CAT#: VS3-FY997)

• Immunogen
• Synthetic peptide derived from human MyoD1.

• Host Species
• Mouse

• Type
• Mouse IgG2a

• Specificity
• Human MYOD1

• Species Reactivity
• Human

• Clone
• 3A10-1C10-10D5

• Applications
• Immunohistochemistry-Paraffin

• Conjugate
• Unconjugated

• Purification
• Affinity Chromatography

• Purity
• >95% as determined by SDS-PAGE

• Buffer
• PBS, pH 7.3, 50% glycerol, 0.5% BSA

• Preservative
• 0.02% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Immunohistochemistry-Paraffin: 1/100-1/500

• Alternative Names
• Myogenic Differentiation 1; Myogenic Factor 3; Class C Basic Helix-Loop-Helix Protein 1; Myoblast Determination Protein 1; BHLHc1; Myf-3; MYF3; MYOD; PUM

• Gene ID
• 4654

• UniProt ID
• P15172

• Cellular Localization
• Nucleus

• Post Translation Modifications
• Phosphorylated by CDK9. This phosphorylation promotes its function in muscle differentiation.
  Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function (By similarity).
  Ubiquitinated on the N-terminus; which is required for proteasomal degradation.
  Methylation at Lys-104 by EHMT2/G9a inhibits myogenic activity.

• Protein Refseq
• NP_002469.2

• Function
• Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity).

Secondary Antibody

• 2ND-048CQ
• Goat Anti-Mouse IgG2a Antibody, R-PE

• 2RD-A1464-YJ
• Goat Anti-Mouse IgG2a Secondary antibody

• 2RD-A1564-YJ
• Rabbit Anti-Mouse IgG2a Secondary antibody (Clone RM219)

Isotype Control

• MOB-202CQ
• Mouse IgG2a, kappa Isotype Control Antibody

Reconstitution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS