Recombinant Mouse Anti-MYOD1 Antibody (clone 3B4C3) (CAT#: VS3-FY996)
This product is a recombinant mouse antibody that recognizes MYOD1. This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Immunocytochemistry, Flow Cytometry. The clone 3B4C3 is specific for MYOD1.
Specific Inquiry
Subcellular Location
RNA Expression
Figure 1 IF staining of human cell line LHCN-M2
(Immunofluorescent staining of human cell line LHCN-M2 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55436/1781_C6_34_selected.jpg
(Immunofluorescent staining of human cell line LHCN-M2 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55436/1781_C6_34_selected.jpg
Figure 2 IF staining of human cell line RH-30
(Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78647/1807_A12_34_selected.jpg
(Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78647/1807_A12_34_selected.jpg
Figure 3 RNA cell line category: Cell line enriched (RH-30)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000129152-MYOD1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000129152-MYOD1
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1
- Clone
- 3B4C3
- Applications
- Enzyme-linked Immunosorbent Assay, Immunocytochemistry, Flow Cytometry
- Conjugate
- Unconjugated
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Immunocytochemistry, Flow Cytometry.
Target
- Alternative Names
- Myogenic Differentiation 1; Myogenic Factor 3; Class C Basic Helix-Loop-Helix Protein 1; Myoblast Determination Protein 1; BHLHc1; Myf-3; MYF3; MYOD; PUM
- Gene ID
- 4654
- UniProt ID
- P15172
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Phosphorylated by CDK9. This phosphorylation promotes its function in muscle differentiation.
Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function (By similarity).
Ubiquitinated on the N-terminus; which is required for proteasomal degradation.
Methylation at Lys-104 by EHMT2/G9a inhibits myogenic activity.
- Protein Refseq
- NP_002469.2
- Function
- Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity).
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "MYOD1"
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2352 | Hi-Affi™ Recombinant Rabbit Anti-MYOD1 Monoclonal Antibody (DS2352AB) | WB, IP, FC | IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1054-CN | Recombinant Rabbit Anti-MYOD1 Monoclonal Antibody (JM10-72) | WB, IP, FC | Rabbit IgG |
| VS3-WK545 | Rabbit Anti-MYOD1 Recombinant Antibody (clone JM10-72) | WB, IP, FC | Rabbit IgG |
| VS3-CJ664 | Mouse Anti-MYOD1 Recombinant Antibody (clone ABT-MYOD1) | WB, IF, IHC-P | Mouse IgG |
| VS3-CJ665 | Mouse Anti-MYOD1 Recombinant Antibody (clone PT2074) | IF, IHC-P | Mouse IgG2a, κ |
| VS3-XY1131 | Mouse Anti-MYOD1 Recombinant Antibody (clone 1C8) | ELISA, WB | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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