Recombinant Mouse Anti-NBR1 Antibody (VS4-CJ49) (CAT#: VS4-CJ49)

• Immunogen
• Recombinant Protein

• Host Species
• Mouse

• Type
• Mouse IgG

• Specificity
• Human, Mouse, Rat NBR1

• Species Reactivity
• Human, Mouse, Rat

• Applications
• ELISA, IHC

• Conjugate
• Unconjugated

• Purification
• Immunogen affinity chromatography

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 50% Glycerol, PBS, pH 7.4.

• Preservative
• 0.02% Sodium Azide

• Storage
• Store at -20°C or -80°C upon receipt. Avoid repeated freeze.

• Application Notes
• This antibody has been tested for use in ELISA, Immunohistochemistry (1:50-1:200).

• Alternative Names
• IAI3B; M17S2; MIG19; 1A1-3B

• Gene ID
• 4077

• UniProt ID
• Q14596

• Long Name
• NBR1 autophagy cargo receptor

• Cellular Localization
• Cytoplasm, Cytoplasmic vesicle, Lysosome

• Post Translation Modifications
• (Microbial infection) Cleaved by S.pyogenes SpeB protease; leading to its degradation (PubMed:24331465).
  Degradation by SpeB prevents autophagy, promoting to S.pyogenes intracellular replication (PubMed:24331465).
  Phosphorylated by GSK3A; this phosphorylation inhibits NBR1 involvement in the formation of ubiquitinated protein aggregates.

• Protein Refseq
• NP_001278500.1; NP_001278501.1; NP_005890.2

• Function
• Ubiquitin-binding autophagy adapter that participates in different processes including host defense or intracellular homeostasis (PubMed:33577621, PubMed:24692539).
  Possesses a double function during the selective autophagy by acting as a shuttle bringing ubiquitinated proteins to autophagosomes and also by participating in the formation of protein aggregates (PubMed:24879152, PubMed:34471133).
  Plays a role in the regulation of the innate immune response by modulating type I interferon production and targeting ubiquitinated IRF3 for autophagic degradation (PubMed:35914352).
  In response to oxidative stress, promotes an increase in SQSTM1 levels, phosphorylation, and body formation by preventing its autophagic degradation (By similarity).
  In turn, activates the KEAP1-NRF2/NFE2L2 antioxidant pathway (By similarity).
  Plays also non-autophagy role by mediating the shuttle of IL-12 to late endosome for subsequent secretion (By similarity).

Isotype Control

• MRO-A1591-YJ
• Mouse IgG Isotype control, Polyclonal

Secondary Antibody

• 2ND-040CQ
• Goat Anti-Mouse IgG (H&L) Antibody

• 2ND-042CQ
• Rabbit Anti-Mouse IgG (H&L) Antibody

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS