Recombinant Rabbit Anti-NEFH Antibody (clone R07-3I6) (CAT#: VS3-FY1024)
This product is a recombinant rabbit antibody that recognizes NEFH. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence. The clone R07-3I6 is specific for human, mouse, rat NEFH. The antigen is human neurofilament heavy chain polypeptide recombinant protein.
Specific Inquiry
Normal Tissue
RNA Expression
Figure 1 High expression in cerebral cortex
(Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7786/20899_B_7_5.jpg
(Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7786/20899_B_7_5.jpg
Figure 2 Colon
(Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7786/20899_A_7_3.jpg
(Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7786/20899_A_7_3.jpg
Figure 3 Liver
(Hepatocytes Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7786/20899_A_9_4.jpg
(Hepatocytes Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7786/20899_A_9_4.jpg
Figure 4 Prostate
(Glandular cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7786/20899_A_1_5.jpg
(Glandular cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7786/20899_A_1_5.jpg
Figure 5 RNA cell line category: Cell line enriched (SiHa)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100285-NEFH
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100285-NEFH
Specifications
- Immunogen
- Human Neurofilament Heavy Chain Polypeptide Recombinant Protein.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat NEFH
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R07-3I6
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 112 kDa; Observed MW: 180-220 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Neurofilament Heavy; Neurofilament, Heavy Polypeptide 200kDa; Neurofilament Heavy Polypeptide; Neurofilament Triplet H Protein; 200 KDa Neurofilament Protein; NF-H; NFH; KIAA0845; CMT2CC
- Gene ID
- 4744
- UniProt ID
- P12036
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "NEFH"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1632z | Mouse Anti-NEFH Recombinant Antibody (clone 27C8) | WB, ELISA, FC, ICC, IF, IHC | Mouse IgG1 |
| MOB-2423CT | Recombinant Mouse anti-Human NEFH Monoclonal antibody (5I9) | WB, IHC-P, IHC-Fr, ICC/IF | |
| MRO-1078-CN | Recombinant Rabbit Anti-NEFH Monoclonal Antibody (JF99-07) | WB, IHC | Rabbit IgG |
| VS3-QX805 | Mouse Anti-NEFH Recombinant Antibody (clone 8H10) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-XY1149 | Mouse Anti-NEFH Recombinant Antibody (clone 2G1) | WB, IP, IF, IHC | Mouse IgG1 |
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0719MZ | Chicken Anti-NEFH Polyclonal IgY | ICC/IF, IHC, WB | Chicken antibody |
| BRD-1121MZ | Chicken Anti-Neurofilament H (NF-H) Polyclonal IgY | WB, IF | Chicken antibody |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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