Recombinant Mouse Anti-NOS3 Antibody (VS3-FY500) (CAT#: VS3-FY500)
This product is a recombinant mouse antibody that recognizes human, mouse and rat NOS3. The eNOS antibody can detects endogenous eNOS protein protein. It can detect a band of approximately 130 kDa.
Specific Inquiry
Normal Tissue
RNA Expression
Figure 1 High expression in spleen.
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2168/ihc_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2168/ihc_selected.jpg
Figure 2 Low expression in cerebral cortex.
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2168/5989_B_7_5_val_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2168/5989_B_7_5_val_selected.jpg
Figure 3 Cerebral cortex
(Glial cells Staining: Not detected Intensity: Weak Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2168/5989_B_7_5.jpg
(Glial cells Staining: Not detected Intensity: Weak Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2168/5989_B_7_5.jpg
Figure 4 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2168/5989_A_7_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2168/5989_A_7_3.jpg
Figure 5 Kidney
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2168/5989_A_9_5.jpg
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2168/5989_A_9_5.jpg
Figure 6 RNA cell line category: Cell line enhanced (BEWO, HUVEC TERT2, K-562, TIME)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000164867-NOS3
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000164867-NOS3
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- The eNOS antibody can detects endogenous eNOS protein.
- Species Reactivity
- Human, Mouse, Rat
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- 130 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS, pH 7.4, 50% Glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western blotting: 1/1000-1/5000
Target
- Alternative Names
- Nitric Oxide Synthase 3; Nitric Oxide Synthase 3 (Endothelial Cell); Constitutive NOS; Endothelial NOS; EC 1.14.13.39; NOS Type III; EC-NOS; NOSIII; CNOS
- Gene ID
- 4846
- UniProt ID
- P29474
- Sequence Similarities
- Belongs to the NOS family.
- Cellular Localization
- Cell membrane, Cytoplasm, Cytoskeleton, Golgi apparatus, Membrane
- Post Translation Modifications
- Phosphorylation by AMPK at Ser-1177 in the presence of Ca2+-calmodulin (CaM) activates activity. In absence of Ca2+-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity).
Phosphorylation of Ser-114 by CDK5 reduces activity.
- Protein Refseq
- NP_000594.2; NP_001153581.1; NP_001153582.1
- Function
- Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway (PubMed:1378832).
NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Recommended Products
Secondary Antibody
Recommended Dilution Buffer
Isotype Control
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "NOS3"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2144z | Mouse Anti-NOS3 Recombinant Antibody (clone 25D5) | WB, ELISA, IF, IHC, IP | Mouse IgG2a, κ |
| MOB-0663CT | Recombinant Mouse anti-Human NOS3 Monoclonal antibody (7I3) | ELISA, IHC-Fr, IHC-P, WB | |
| MRO-1840-CN | Rabbit Anti-NOS3 Polyclonal Antibody (MRO-1840-CN) | IHC | Rabbit IgG |
| VS3-XY1172 | Mouse Anti-NOS3 Recombinant Antibody (clone 6H2) | ELISA, IHC | Mouse IgG1 |
| VS3-WK1419 | Mouse Anti-NOS3 Antibody (clone Mix-mA) | WB | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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