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  • Recombinant Mouse Anti-POU5F1 Antibody (VS-0923-FY125)

Recombinant Mouse Anti-POU5F1 Antibody (VS-0923-FY125) (CAT#: VS-0923-FY125)

The VS-0923-FY125 is a murine IgG1 antibody that binds to POU5F1. The epitope of this antibody is within the amino acids region 102-265 of human POU5F1.


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Specific Inquiry
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  • Datasheet
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line RT4
Figure 1 IF staining of human cell line RT4
(Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58267/1518_H6_1_selected.jpg
Figure 2 IHC staining of human hippocampus
Figure 2 IHC staining of human hippocampus
(Immunohistochemical staining of human hippocampus shows strong nuclear positivity in glial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25600/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Figure 3 Cerebral cortex
(Glial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_B_8_5.jpg
Figure 4 Colon
Figure 4 Colon
(Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_7_3.jpg
Figure 5 Liver
Figure 5 Liver
(Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_7_4.jpg
Figure 6 Pancreas
Figure 6 Pancreas
(Exocrine glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Pancreatic endocrine cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_3_3.jpg
Figure 7 Kidney
Figure 7 Kidney
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_8_5.jpg
Figure 8 Testis
Figure 8 Testis
(Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25600/54340_A_6_6.jpg
Figure 9 Lymph node
Figure 9 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_8_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enriched (SuSa)
Figure 10 RNA cell line category: Cell line enriched (SuSa)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000204531-POU5F1/subcellular

Specifications

  • Immunogen
  • POU5F1 protein fragment (a.a. 102-265)
  • Host Species
  • Mouse
  • Type
  • Mouse IgG1
  • Specificity
  • Human POU5F1
  • Species Reactivity
  • Human
  • Applications
  • ELISA, Western Blot
  • Conjugate
  • Unconjugated

Product Property

  • Format
  • Liquid
  • Buffer
  • Citrate Tris HCl buffer
  • Preservative
  • 0.02% Proclin 300
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot (1/1000)
    Specific conditions should be optimized by the end user.

Target

  • Alternative Names
  • NF-A3, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4#

Recommended Products

Isotype Control

Secondary Antibody

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "POU5F1"

Recombinant Antibody

CAT Product Name Application Type
MOB-2215MZ Recombinant Mouse Anti-Human POU5F1 Antibody (clone OSH2.2) IHC-P Mouse antibody
MRO-1126-CN Recombinant Rabbit Anti-POU5F1 Monoclonal Antibody (CBACN-411) WB, IF, IHC, IP, ChIP Rabbit IgG
MRO-1127-CN Recombinant Mouse Anti-POU5F1 Monoclonal Antibody (A3-C11) WB, IF Mouse IgG1
MRO-2092-CN Rabbit Anti-POU5F1 Polyclonal Antibody (MRO-2092-CN) WB, IF, IHC, FC Rabbit IgG
VS3-CJ41 Mouse Anti-POU5F1 Recombinant Antibody (VS3-CJ41) WB, ELISA Mouse IgG

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-2797 Hi-Affi™ Recombinant Rabbit Anti-POU5F1 Monoclonal Antibody (DS2797AB) FC, IHC-P, IP, WB IgG
MOR-0065-FY Rabbit Anti-POU5F1 Recombinant Antibody (clone AFY0036) FC, ICC, IHC-P, WB Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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