Recombinant Mouse Anti-S100A1 Antibody (clone 9E8-8B8-9B4) (CAT#: VS3-FY1292)

• Immunogen
• Synthetic peptide derived from human S100.

• Host Species
• Mouse

• Type
• Mouse IgG1

• Specificity
• Human S100A1

• Species Reactivity
• Human

• Clone
• 9E8-8B8-9B4

• Applications
• Immunohistochemistry-Paraffin

• Conjugate
• Unconjugated

• Purification
• Affinity Chromatography

• Purity
• >95% as determined by SDS-PAGE

• Buffer
• PBS, pH 7.3, 50% glycerol, 0.5% BSA

• Preservative
• 0.02% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Immunohistochemistry-Paraffin: 1/100-1/500

• Alternative Names
• S100 Calcium Binding Protein A1; S100 Calcium-Binding Protein A1; S-100 Protein Subunit Alpha; S-100 Protein Alpha Chain; S100A; S100 Protein, Alpha Polypeptide

• Gene ID
• 6271

• UniProt ID
• P23297

• Sequence Similarities
• Belongs to the S-100 family.

• Cellular Localization
• Cytoplasm, Mitochondrion, Sarcoplasmic reticulum

• Post Translation Modifications
• Glutathionylated; glutathionylation increases affinity to calcium about 10-fold.

• Protein Refseq
• NP_006262.1

• Function
• Small calcium binding protein that plays important roles in several biological processes such as Ca2+ homeostasis, chondrocyte biology and cardiomyocyte regulation (PubMed:12804600).
  In response to an increase in intracellular Ca2+ levels, binds calcium which triggers conformational changes (PubMed:23351007).
  These changes allow interactions with specific target proteins and modulate their activity (PubMed:22399290).
  Regulates a network in cardiomyocytes controlling sarcoplasmic reticulum Ca2+ cycling and mitochondrial function through interaction with the ryanodine receptors RYR1 and RYR2, sarcoplasmic reticulum Ca2+-ATPase/ATP2A2 and mitochondrial F1-ATPase (PubMed:12804600).
  Facilitates diastolic Ca2+ dissociation and myofilament mechanics in order to improve relaxation during diastole (PubMed:11717446).

Secondary Antibody

• 2ND-047CQ
• Goat Anti-Mouse IgG1 Antibody, R-PE

• 2RD-A1460-YJ
• Goat Anti-Mouse IgG1 Secondary antibody

Isotype Control

• MOB-065CQ
• Mouse IgG1 Isotype Control Antibody

Reconstitution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS