Recombinant Rabbit Anti-S100A9 Antibody (clone R05-3C3) (CAT#: VS3-FY1299)
This product is a recombinant rabbit antibody that recognizes S100A9. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence. The clone R05-3C3 is specific for mouse S100A9. The antigen is mouse s100a9 recombinant protein.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/33_B12_2_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/33_B12_2_selected.jpg
Figure 2 Cerebral cortex
(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/13990_B_8_5.jpg
(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/13990_B_8_5.jpg
Figure 3 Esophagus
(Squamous epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/13990_B_6_1.jpg
(Squamous epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/13990_B_6_1.jpg
Figure 4 Colon
(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/13990_A_9_3.jpg
(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/13990_A_9_3.jpg
Figure 5 RNA cell line category: Cell line enriched (SK-BR-3)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000163220-S100A9
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000163220-S100A9
Specifications
- Immunogen
- Mouse S100A9 recombinant protein.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Mouse S100A9
- Species Reactivity
- Mouse
- Clone
- R05-3C3
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 13 kDa; Observed MW: 13 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- S100 Calcium Binding Protein A9; Migration Inhibitory Factor-Related Protein 14; Leukocyte L1 Complex Heavy Chain; Calprotectin L1H Subunit; MRP-14; MRP14; CFAG; CAGB; P14; S100 Calcium-Binding Protein A9 (Calgranulin B); S100 Calcium Binding Protein A9 (Calgranulin B); S100 Calcium-Binding Protein A9
- Gene ID
- 6280
- UniProt ID
- P06702
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "S100A9"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1868z | Mouse Anti-S100A9 Recombinant Antibody (clone 10B3) | WB, ELISA, IHC | Mouse IgG2b |
| MOB-1452MZ | Recombinant Mouse Anti-Human S100A9 Antibody (clone OP.245) | ELISA, sELISA | Mouse antibody |
| HPAB-0323-CN | Mouse Anti-S100A9 Recombinant Antibody (clone IID4B10) | WB, ELISA, IHC | Mouse IgG1, κ |
| MRO-1359-CN | Recombinant Rabbit Anti-S100A9 Monoclonal Antibody (JF096-8) | WB, IF, IHC | Rabbit IgG |
| ZG-0160U | Mouse Anti-S100A9 Recombinant Antibody (clone 1G1C1) | ELISA, IHC | Mouse IgG2b |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3159 | Hi-Affi™ Recombinant Rabbit Anti-S100A9 Monoclonal Antibody (DS3159AB) | ICC, IHC-P, WB | IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0323-CN-S(P) | Mouse Anti-S100A9 Recombinant Antibody (clone IID4B10); scFv Fragment | WB, ELISA, IHC | Mouse scFv |
| HPAB-2539LY-S(P) | Mouse Anti-S100A9 Recombinant Antibody; scFv Fragment (HPAB-2539LY-S(P)) | ELISA, Block | Mouse scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0323-CN-F(E) | Mouse Anti-S100A9 Recombinant Antibody (clone IID4B10); Fab Fragment | WB, ELISA, IHC | Mouse Fab |
| HPAB-2539LY-F(E) | Mouse Anti-S100A9 Recombinant Antibody; Fab Fragment (HPAB-2539LY-F(E)) | ELISA, Block | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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