Recombinant Rabbit Anti-S100A9 Capture Antibody (clone R19-5C-9) (CAT#: VS3-WK1926)
This antibody is a rabbit recombinant antibody against human S100A9. It was developed by immunization of rabbit with recombinant human S100A9 protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. The sandwich ELISA was established using matched antibody pair R19-5C-9 and R31-4D-5. The clone R19-5C-9 can be used as a capture antibody in ELISA analysis to identify human S100A9.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/33_B12_2_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/33_B12_2_selected.jpg
Figure 2 Cerebral cortex
(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/13990_B_8_5.jpg
(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/13990_B_8_5.jpg
Figure 3 Esophagus
(Squamous epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/13990_B_6_1.jpg
(Squamous epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/13990_B_6_1.jpg
Figure 4 Colon
(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/13990_A_9_3.jpg
(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4193/13990_A_9_3.jpg
Figure 5 RNA cell line category: Cell line enriched (SK-BR-3)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000163220-S100A9
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000163220-S100A9
Specifications
- Immunogen
- Recombinant human S100A9 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human S100A9
- Species Reactivity
- Human
- Clone
- R19-5C-9
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01M PBS, pH 7.2
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- S100 Calcium Binding Protein A9; Migration Inhibitory Factor-Related Protein 14; Leukocyte L1 Complex Heavy Chain; Calprotectin L1H Subunit; MRP-14; MRP14; CFAG; CAGB; P14; S100 Calcium-Binding Protein A9 (Calgranulin B); S100 Calcium Binding Protein A9 (Calgranulin B); S100 Calcium-Binding Protein A9
- Gene ID
- 6280
- UniProt ID
- P06702
- Long Name
- S100 Calcium Binding Protein A9
- Sequence Similarities
- Belongs to the S-100 family.
- Cellular Localization
- Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Secreted
- Post Translation Modifications
- Phosphorylated. Phosphorylation inhibits activation of tubulin polymerization.
S-nitrosylation of Cys-3 is implicated in LDL(ox)-induced S-nitrosylation of GAPDH at 'Cys-247' through a transnitrosylase mechanism involving a iNOS-S100A8/9 complex.
Methylation at His-105 by METTL9 reduces zinc-binding without affecting heterodimerization with S100A8.
- Protein Refseq
- NP_002956.1
- Function
- S100A9 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response.
Recommended Products
Detection antibody
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "S100A9"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1452MZ | Recombinant Mouse Anti-Human S100A9 Antibody (clone OP.245) | ELISA, sELISA | Mouse antibody |
| HPAB-0323-CN | Mouse Anti-S100A9 Recombinant Antibody (clone IID4B10) | WB, ELISA, IHC | Mouse IgG1, κ |
| MRO-1359-CN | Recombinant Rabbit Anti-S100A9 Monoclonal Antibody (JF096-8) | WB, IF, IHC | Rabbit IgG |
| HPAB-2539LY | Mouse Anti-S100A9 Recombinant Antibody (HPAB-2539LY) | ELISA, Block, WB | Mouse IgG |
| VS-0224-XY108 | Rat Anti-S100A9 Recombinant Antibody (clone 2B10) | IF, IHC-P, ICC | Rat IgG2a |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3159 | Hi-Affi™ Recombinant Rabbit Anti-S100A9 Monoclonal Antibody (DS3159AB) | ICC, IHC-P, WB | IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0323-CN-S(P) | Mouse Anti-S100A9 Recombinant Antibody (clone IID4B10); scFv Fragment | WB, ELISA, IHC | Mouse scFv |
| HPAB-2539LY-S(P) | Mouse Anti-S100A9 Recombinant Antibody; scFv Fragment (HPAB-2539LY-S(P)) | ELISA, Block | Mouse scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0323-CN-F(E) | Mouse Anti-S100A9 Recombinant Antibody (clone IID4B10); Fab Fragment | WB, ELISA, IHC | Mouse Fab |
| HPAB-2539LY-F(E) | Mouse Anti-S100A9 Recombinant Antibody; Fab Fragment (HPAB-2539LY-F(E)) | ELISA, Block | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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